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Results: The cultured cells showed typical shape and features of fibroblast cell ,which were successfully dissociated and purified.

结果:原代培养的成纤维细胞特征典型,并通过传代培养得到了纯化的成纤维细胞。

The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板,NS5B1和NS5B2为聚合酶,进行体外的RNA合成反应,斑点杂交和RT-PCR检测产物,确定纯化的蛋白具有RdRp活性。

The purified proteins had RdRp activity, and could synthesize RNA products similar in length to the RNA templates using the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand.

纯化的NS5B蛋白具有体外RdRp活性,能够以HCV负链3'末端RNA为模板体外合成一条与模板大小相近的产物。

All the purified scFvs had the antigen-binding activity by ELISA and could be further used for immunoassay and disease control for citrus bacterial canker disease.

ELISA检测纯化的单链抗体都具备抗原结合活性,可进一步应用于柑桔溃疡病菌的免疫诊断和病害防治。

There are, however, no specific Wnt agonists and no method for in vivo delivery of purified Wnt proteins.

然而,没有特异的Wnt激动剂,也没有办法在体内释放纯化的Wnt蛋白。

The integrations between the episome DNA and the Dd2 genomic DNA at the sites of HRP3-5'and HRP2-3' were identified by the PCR analysis after 7 weeks of transformation but that of the site of Pfdyn gene was not identified.

在恶性疟原虫体外抑制实验中,纯化的抗Pfdyn-N IgG可以在体外有效地抑制恶性疟原虫Dd2和FCC1虫株的生长,抑制率分别达92.6%和91.8%。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清,将纯化的抗磷酸酪氨酸抗体作为第一抗体,羊抗兔Ig酶结合物作为第二抗体,建立双抗体夹心酶联免疫吸附测定法。

Objective To investigate the molluscicidal activities of the ginkgolic acid monomers isolated and purified from GAs.

摘要 目的探索分离纯化的银杏酸同系物单体对钉螺的影响。

The specific binding ability of these IgY antibodies to SARS-CoV-infected cells was demonstrated by immunofluorescence assay.

在免疫萤光染色法中可见纯化的IgY与被SARS-CoV感染的细胞具有特异性结合反应。

Based on the above statistical analyses, the study infers that proterozoic Carassius auratus Tungting from Beiming Lake have the property of high pureness.

洞庭青鲫亲子间各性状统计分析说明,子一代不仅没有性状分离现象,而且基因有进一步纯化的趋势。由此推论:北民湖洞庭青鲫原种种群的纯度较高。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。