纯化的

The Schizosaccharomyces pombe plasma membrane H(superscript +)-ATPases are obtained by differential centrifugation, acid treatment and isopycnic centrifugation in a discontinuous sucrose gradient to study the effects of Ca(superscript 2+), CaM and CaM inhibitors on activity of purified plasma membrane H(superscript +)-ATPase.

经差速离心，酸处理，蔗糖密度梯度离心制备粟酒裂殖酵母质膜H-ATP酶，以研究Ca(上标 2+)、CaM以及CaM拮抗剂对纯化的质膜H-ATP酶活性的影响。

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后，取脾细胞和SP2/0骨髓瘤细胞进行融合后，以间接ELISA和有限稀释法进行筛选，获得4株能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞株，该株单克隆抗体命名为1F2，2C2，3D6，4H6株。

Three-hundred and ninety-nine fungal isolates were obtained from 686 diseased cotton seedlings and soil samples collected from cotton production areas in Northern Xinjiang by using the conventional method,among which 272 strains of R.solani were identified.Based on Giemsa staining,all strains were identified as multinucleate Rhizoctonia solani .

从新疆北疆棉区采集了典型的棉花立枯病病苗及棉田土标样686份，按常规分离方法分离得到399个分离物，从中鉴定出272个纯化的立枯丝核菌( Rhizoctonia solani Kühn)菌株。

To study the effect of natrahagin, a Chinese cobra venom proteinase purified from Naja naja atra Z venom on platelet aggregation,fibrinogen level and artery thrombosis.

研究从中国黑眼镜蛇毒中纯化的中国黑眼镜蛇毒蛋白酶natrahagin对血小板聚集，血浆纤维蛋白原水平和动脉血栓形成的影响。

To observe the growth inhibitory effects of 10 peptides (KD Ⅰ-1, KD Ⅰ-1 H, KD Ⅰ-2, KD Q Ⅰ-2, KD Ⅱ, KD Ⅱ-2, KD Ⅱ-3, KD Ⅲ-1, KD Ⅲ-2, KD Ⅲ-2) purified from KD, one of components in Naja Naja Actra venom, on human glioma U251 cell line.

目的：探讨中华眼镜蛇毒卡迪中进一步分离纯化的10个组分KDⅠ-1、KDⅠ-1 H、KDⅠ-2、KDQⅠ-2、KDⅡ、KDⅡ-2、KDⅡ-3，KDⅢ-1，KDⅢ-2和KDⅢ-2′对神经胶质瘤细胞U251的抑制作用。

To study furderly the activity of CNTF mutant designed by computer molecular modling,this study used the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice′weight loss tests.

为了进一步研究我室应用计算机分子模拟设计并表达纯化的睫状神经营养因子突变体蛋白的生物学活性，分别采用鸡胚背根神经节无血清培养法、TF-1细胞增殖法、正常小鼠减重法对其活性进行研究。

Using the thermostable ability of Taq DNA polymerase, the protein was immerged in a 75℃ water-bath for 1h, and then obtained by the method of ammonium sulfate precipitation.

利用Taq酶的热稳定性，将破胞液在75℃水浴1h，然后用硫酸铵沉淀法获得纯化的表达蛋白。

T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使纯化的生物分子结晶为有序排列然后用X射线分析结晶体。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失