纯化的

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

Methods: the cells are isolated by two-step enzyme digestion and purified by the Percoll density centrifugation,the proliferation and differentiation propertiesof which being observed.To study the effect of IGF-1 on satellite cell with MTT method and obtain adequate single satellite cell by frequent passaging.

运用两步酶消化法分离卫星细胞，不连续密度梯度离心法纯化后体外培养，观察其生长与分化特性，运用MTT法测定卫星细胞对不同浓度IGF刺激的反应，并运用频繁传代的方法获取足量单个肌卫星细胞。

These techniques are mostly based on the use of enzymes purified from bacteria and animal cells, that allow steps such as DNA copying, conversion into RNA, and into protein, to be recapitulated in the laboratory.

这些技术主要基于对从细菌和动物细胞里纯化出的酶的使用，使得DNA复制、转化为RNA、转译为蛋白质都可以在实验室完成。

To purify platelets by magnetic activated cell soring LD and MS respcctively. To evaluate karyotc clearance by leucocount method and to detect the platelets activation grade by flow cytometry method.

血细胞分离机单采外周血中的血小板，分别采用磁性细胞MS柱和LD柱纯化浓缩血小板，采用白细胞绝对计数法对分选前后血小板中的有核细胞进行检测，计算有核细胞去除率。

Equipment used for purification, separation or concentration should be sterilised or disinfected at least between use for different products.

用于纯化，分离和浓缩的设备，至少在进行两个产品生产之间的更换时，要进行消毒和灭菌。

The synthetic procedure consists of three steps: the preparation of magnetic silica microbeads by polymer templating method; coupling of iminodiacetic acid with a bifunctional silane agent 3-glycidoxypro-pyltrimethoxysilane; charging the magnetic support with Cu(superscript 2+) With bovine serum albumin as a model compound, the adsorption behavior of protein on the magnetic matrix was studied.

采用模板法制备的单分散磁性硅胶微球，经过表面修饰偶联上亚氨基二乙酸，与过渡金属离子Cu(上标 2+)螯合，制成一种新型的磁性固定化金属亲和纯化载体。

Results The molecular relative mass of immunocytokine, IP10-scFv is 41.1 kD, affinity constant of IP10-scFv is 2.28×10^-8 with Scatchard formula.

结果重组免疫细胞因子（IP10-scFv）真核表达产物的相对分子质量约为41.1 10^3，纯化后的蛋白浓度为68mg／L，抗体亲和常数（KDIP10-scFv）为2.28 10^-8mol／L。

Methodspolysaccharides a was extracted and purified through 0.5% ammonium oxalate and ethanol precipitation, the content was determined by phenol-sulfuric acid under 485 nm.

方法采用0.5%草酸铵溶液提取、醇沉的方法分离纯化大蒜多糖组分a，以苯酚-硫酸显色法测定大蒜多糖组分a的含量。

For ammonium sulphate precipitation, ammonium sulphate with different concentrations was added to culture filtrate.

再后，论文根据现有实验室的实验条件，选择硫酸铵盐析、凝胶层析和CHT层析对角蛋白酶的粗酶进行了纯化。

This paper study compose of coconut water protein by SDS-PAGE gel electrophoresis. The results showed that there were nine subunits in coconut water protein.

对椰子水中的蛋白质进行分离纯化，利用SDS-PAGE凝胶电泳系统，对椰子水中蛋白的结构进行了分析，结果表明，在椰子水蛋白质中，含有9个亚基结构。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失