纯化的

Purified GO could oxidize both glycolate and glyoxylate, and both activities highly depended on FMN. The activity of glyoxylate oxidation was weaker than that of glycolate. During purification or storage of purified GO, the ratio of glycolate to glyoxylate oxidation was progressively enhanced.

纯化的菜心叶片乙醇酸氧化酶对乙醇酸和乙醛酸均有氧化活性，且两者都强烈依赖于FMN，但对乙醛酸的氧化能力相对较弱；在酶的纯化及纯化酶的贮存过程中其两催化活性的比值均呈逐渐升高的趋势。

Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶，比活性达130.9 U／mg，纯化倍数为137.4倍；纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰，且两者完全重叠；进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色，各在负极端出现一个活性斑点和蛋白染色斑点，且两者的位置完全对应；表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

Yes, because, basically, you're bringing in the active ingredient in an unpurified form and subjecting it to the purification.

因为，你们基本上购买了未纯化的活性物质将它纯化。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

The results indicated that the purity and immunoactivity varied according to different purification methods. IgG purified by caprylic acid precipitation plus ammonium sulfate precipitation is the best for further use with 98.05%purity and 1800 ratio activity.

结果表明，不同方法纯化的抗体其纯度和免疫学活性具有一定程度的差别，其中经辛酸+饱和硫酸铵沉淀纯化的抗体为最佳，凝胶扫描纯度为98.05%，比活性近1 800，为纯化前的6.8倍。

Objective: To choose the optimum resin for separation and purification of mistletoe flavonoids and to analyze influenceable factors of separation and purification process.

目的：筛选出分离纯化槲寄生总黄酮的最佳树脂，并对影响分离纯化的因素进行研究，得到优化的纯化条件。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

Affinity chromatography materials for kinase;2. To search a suitable process route of purification of kinase in yeast by affinity chromatography, hexokinase was preparatorily purified by salt preciptation.

为确定一条采用亲和层析技术分离纯化酵母中磷酸激酶的较佳工艺路线，采用分步盐析初步纯化己糖激酶，考察硫酸铵饱争度对盐析效果的影响；以ATP为配基，SESA活化纸纤堆素制备亲和层析剂，确定最佳偶联条件和最佳静态吸附pH值后，将粗酶液直接上亲和层析柱，看分离纯化的效果；在载体和配基之问接上1。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

The objective was to develop a rapid and simple colloidal gold immunochromatographic assay for the diagnostic of chicken NDV. Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol. Colloidal golds were coupled with the purified anti-ND antibody.

为了建立一种快速、简便的检测鸡新城疫病毒的胶体金免疫层析法，采用柠檬酸钠还原法制备胶体金颗粒，标记纯化的ND抗体，并包被在玻璃纤维素膜上，另外将纯化的ND抗体和纯化的兔抗鸡抗体包被在硝酸纤维素膜上，组装成ND快速检测试纸条。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失