纯化

Echistatin is a polypeptide with 4pairs of disulfide bridges isolated from Echis carinatus. Its isoelectric point is 8. 3; its molecular weight is 5400kD.

蛇毒蛋白锯鳞蝰素是从小蝰属锯鳞蝰的蛇毒中纯化出的，等电点pI为8.3，有四对二硫键，分子量为5400道尔顿的单链多肽。

Echistatin is a polypeptide with 4pairs of disulfide bridges isolated from Echis carinatus.

蛇毒蛋白锯鳞蝰素是从小蝰属锯鳞蝰的蛇毒中纯化出的，等电点pI为8.3，有四对二硫键，分子量为5400道尔顿的单链多肽。。。。

Freeing itself from the sensuous world by purification, the human soul ascends by successive steps through the various degrees of the metaphysical order, until it unites itself in a confused and unconscious contemplation to the One, and sinks into it: it is the state of ecstasis.

解放自己从感性世界的纯化，人的灵魂ascends历届步骤，通过不同程度的形而上的秩序，直到它本身的团结在一个混乱和昏迷沉思向一，和汇到它：它是国

ABSTRACT Hairy and Enhancer-of-split-type protein 1 (Hes1) is a main effecter of the Notch pathway and acts as a differentiation repressor and/or as a pro-expansion factor during the pancreatic development.

由于人们尚未找到胰腺干细胞的特异性标志，因而给胰腺干细胞的分离纯化及其特性的研究带来了极大的困难。来自hes1-/-敲除小鼠的研究提示，Hes1作为Notch信号通路的一个关键分子，在胰腺发育过程中扮演着扩增促进因子或分化抑制因子的角色，因而有可能用作胰腺干细胞的备选标志分子，但这一推论缺乏直接的证据。

To identity the strains of Eimeria conserved in our laboratory are purebred,11 strains of 6 Eimeria species were separated by single-oocyst method and infected chicken of 1—5 days old.

为了建立鸡球虫纯株，选用1~5日龄雏鸡，采用琼脂块单卵囊分离法对实验室保存的6种11株鸡球虫进行纯化，并选用2周龄雏鸡，对每个虫种/株选取1个单卵囊分离物进行增殖和种类鉴定。

The studies on separation of antibacterial substances and the identification by GC-MS indicate that: Eleocharis Tuberoses peel contains cholesterol, organic acids, eaters and flavonoids four types of active ingredients, and 11kinds of active material.

对荸荠皮抑菌物质纯化后进行的GC-MS鉴定结果显示：荸荠皮抑菌物质中含有甾醇类、有机酸类、酯类和黄酮类4类活性成分共11种活性物质。

The process of extraction of antimicrobial substance from Eleocharis tuberose peel with ultrasonic were studied; the properties of antibacterial substances were studied; antibacterial substances was isolated by TLC and silica gel colum chromatography, and the structure of antibacterial substances were identified useing liquid chromatograph mass spectrometer.

包括：采用超声波法提取荸荠皮抑菌物质并获得最佳工艺条件；研究了荸荠皮提取物的稳定性及其对多种菌的抑制作用；通过薄层层析和硅胶柱层析对抑菌物质分离纯化后对抑菌成分进行了初步的结构鉴定。

This process hydrolyzed ellagitannins effectively and increased ellagic acid content from 2.3 mg/g to 14.9 mg/g.

此制程的终产物鞣花酸纯度可达80%以上，整体纯化过程鞣花酸的回收率约47%。

Further purification was performed on a column of Q-sepharose FF, and the 50mM elute contained the active DM650 with a purity of about 95%.

复性后的蛋白用Q-Sepharose FF离子交换层析进行纯化，50mM NaCl洗脱峰中含有目的蛋白，纯度可达95％。

Using NaCl and Na2HPO4 in turn to elute bound alkaline phosphatase, a 65-fold purified enzymatic preparation was obtained in one step with a yield of 89%.

用NaCl和Na2HPO4进行阶段洗脱，可使酶的纯化倍数一步达到65倍，活力回收率达到89%。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失