纯化

Though their primary structure are different with their species,they all have rich proline exists in N-terminal and 6-Cys locates in 22,23,25,35,44 and 54.Three intrachain disulphide bonds have been found in porcine intestine peptide.

尽管因种属不同在一级结构上略有差异，但其N端脯氨酸密集，22、23、25、35、44和54位点为6个半胱氨酸，从猪小肠纯化肽的3对链内二硫桥键已定位。

Though their primary structure are different with their species,they all have rich proline exists in N-terminal and 6-Cys locates in 22,23,25,35,44 and 54.Three intrachain disulphide bonds have been found in porcine intestine peptide.

尽管因种属不同在一级结构上略有差异，但其N端脯氨酸密集，22、23、25、35、44和54位点 6个半胱氨酸，从猪小肠纯化肽的3对链内二硫桥键已定位。

Fresh algal extracts prepared in 0.5 M HCl were neutralised, purified on a SPE column (RP-18, Merck), lyophilised in the presence of 1 mM dithiothreitol to prevent the formation of disulphide bridges and stored at -/20℃.

食藻提取物中拟定0.5米盐酸被瓦解，纯化的固相萃取柱（RP-18， Merck），lyophilised，在场的1毫米二硫苏糖醇，以防止形成二硫键桥梁和储存在-/20℃。

As a part of a search for new antitumor agents from Chinese medicinal plants, the roots of Salvia prionitis, collected in Jiangxi Province, was investigated. From the roots of this plant, a new diterpene, saprirearine (95), a new anhydride-type compound, saprionide (96), a new 7, 8-seco-abietane diterpene derivative, 7, 8-seco-paraferruginone (99), and a new 4, 5- seco-5, 10-friedo-abietane diterpenoids, 3-keto-4-hydroxysaprorthoquinone (102), have been isolated along with 6 known compounds. Their structures were established by spectroscopic methods and chemical transformation.

在对红根草的乙酸乙酯提取物进行分离，纯化和结构研究中，共得到10个化合物，其中4个是新化合物，3个新化合物具有新颖的重排松香烷型二萜骨架，新化合物99为首次从本属植物中分得的7，8-开环的松香烷型二萜醌类化合物。

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶，通过优化体系中各底物的添加量，实现荧光素酶的体外发光。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

According to different acidity of free Flavonoids in Resina Draconis, different acidic lavonoids were extracted with the pH gradient extraction method from ethyl ether.

根据血竭中游离黄酮存在着酸性差异，采用溶剂除杂，转溶于乙醚，再用pH梯度萃取得到不同酸性的黄酮，进而对弱酸性组分分离纯化得到2个单体化合物。

The results showed that the-epicatechin-3-O-β-D-allopyranoside extracted from the rhizoma of Drynaria fortunei J.

本实验将从槲蕨根茎经纯化分离出之-epicatechin-3-O-β-D-allopyranoside，已於本实验室证明对骨母细胞有促进增生的作用。

Methods The constituents in the marcroporous adsorption resin 50% EtOH eluent of Drynaria fortunei were isolated by a combination of silica gel, Sephadex LH-20 and ODS chromatographies and their structures were identified by spectral methods.

利用硅胶、Sephadex LH-20、ODS柱色谱及制备型HPLC等方法进行分离纯化，根据化合物的理化性质及波谱数据鉴定结构。

And purification of street perpendicular to a section called dyehouse street alley, there are several special flavored barbecue main course grilled ribs, ribs tender, delicious, very biting head, although many are baked barbecue ribs, but this is my home favorite.

与纯化街垂直有条小街叫染房胡同，有几家烧烤特有味，主菜是烤熟筋，筋嫩，味美，很有咬头，虽然很多烧烤都烤筋，但这家的是我最爱吃的。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失