纯化

TLC analysis demonstrated that highly purified HDC protein was able to decarboxylate histidine to form histamine Fig.

薄层色谱分析表明，HDC的高度纯化的蛋白质能够decarboxylate组氨酸形成组胺（图2bii）。

Sucrose sugars are treated with specialty activated carbons to decolorize and purify.

蔗糖则采用特制的活性炭产品进行脱色和纯化。

The ethanol extract was dissolved with HCl, the acidic extract was basified to pH9-10 with NH_3-water and extracted with CHCl_3 to give total alkaloids. five alkaloids were isolated with column chromatography and preparative thin-layer chromatography, and three of them were identified as dehydrocorydaline、 corynoline and dihydrosanguinarine on the basis of spectral analysis MS,~1H-NMR,~(13C-NMR and physicochemical properties. All of them were found for the first time from this plant.

为研究灰绿黄堇的化学成分，本文将灰绿黄堇乙醇提取物采取酸提取、碱游离后萃取的方法得到总生物碱，然后利用柱色谱、制备薄层色谱等方法分离、纯化了灰绿黄堇中5种生物碱，并利用IR、MS、~1H-NMR、~(13)C-NMR等方法鉴定了其中的3种，分别为去氢紫堇碱、紫堇灵、二氢血根碱，均为首次在此植物中发现。

METHODS: The model of tissue damaged submandibular gland in 10 rats was made by deligation. One week later, the gland tissue was obtained to harvest submandibular gland stem/progenitor cells by enzyme digestion in vitro. Following 10-14 days of primary culture, small round cells were collected, purified and subcultured for monoclonal culture.

10只大鼠采用结扎下颌下腺主导管、抑制腺体分泌来建立组织损伤模型，1周后切取腺体组织，酶消化法体外分离下颌下腺干/祖细胞，原代培养10~14 d后，挑取培养皿中形成的小的类圆形、类上皮细胞集落予以纯化，传代后进行单克隆培养。

The fungi was isolated and purified from polluted anthurium subculture medium, identified to be Deuteromycotina Hyphpmycetes Hyphomycetales Dematiaceae and trichoderma. The inhibition effect of the three kinds of chitosan on the fungi was conducted.

在污染的红掌继代培养基中提取污染菌并进行了分离及纯化，初步鉴定为为半知菌亚门，丝孢纲，丝孢目，淡色孢科，木霉属。

Plant Virology 2 S T. H. Chen, FThe course will give conventional and molecular level of plant viruses in order to help the students to get much theoretical and practical knowledge about plant virology. The contents cover the molecular structure of virion and genome, virus isolation, dentification and purification, transmission, variation,ecology, nomenclature and classification, diagnosis and control.

本课程除传统植物病毒学范畴外，并涵盖从分子层次讨论植物病毒之构造，引起之植物病害，田间发生及防治，期能达到理论与实务并重；内容包括植物病毒分子层次的构造及核酸组成，病毒分离、鉴定与纯化，在寄主体内之增殖及转移，媒介传播、遗传及变异，其引起之病害及田间发生生态与防治。

BACKGROUND: Chitosan and its derivant are environmental-friend natural macromolecular materials, characterizing by great flocculation and adsorption capacities caused by electrophilic group including amino and hydroxyl groups, as well as its long-chain molecular structure. So chitosan is widely used as flocculants in domains of sewage disposal, food refining as well as Chinese medicine purification.

背景：壳聚糖及其衍生物是环境友好的天然高分子材料，其分子中丰富的带电基团如氨基、羟基以及长链分子结构赋予其优良的絮凝吸附能力，被作为絮凝剂广泛用于工业用水、食品精制以及中药液纯化等领域。

During the incubation of purified α-synuclein with fructose or glucose, it was observed that the protein intrinsic fluorescence at 308 nm decreased while the fluorescence of glycated derivant at 447 nm increased.

将纯化后的α-synuclein分别与果糖和葡萄糖孵育，通过内源荧光、非酶糖基化衍生物特征荧光、圆二色光谱以及电子显微镜等技术进行检测发现：α-synuclein 与还原糖共同孵育后，308 nm内源荧光强度明显降低，同时在447 nm产生一个非酶糖基化衍生物特征荧光。

It is concluded that the ultrafiltration preconcentration combining with multieffect evaporation is a promising concentration procedure for straw black liquor, which can not only reduce the concentration costs, but also improve the subsequent evaporation and combustion operation due to the desilicification and purification of ultrafiltration.

两种膜比较，磺化聚砜膜更易清洗，而且水通量大。5）超滤浓缩后，黑液中木素的浓度和纯度大大提高，超滤是从黑液中分离木素的理想方法。6）与多效蒸发相比，用超滤法浓缩麦草黑液可降低能耗70~85%，若用于黑液的预浓缩，再结合多效蒸发，不仅可以降低黑液的浓缩费用，而且由于除硅和纯化作用，会大大改善后继的蒸发和燃烧的操作。

Changes of DBE activity of four cultivars had a single-peak curve, and the peak appeared in 14 d after anthesis. HPLC analysis showed that the activity of DBE to amylopectin was similar. There are two types of DBE in the wheat grains, one is isoamylase, and the other is limit dextrinase.

在灌浆过程中，淀粉去分支酶的活性均呈单峰曲线变化，灌浆中期活性最大；淀粉去分支酶对底物支链淀粉均具有相似的酶解活性；对灌浆中期小麦淀粉去分支酶进行的纯化结果表明，小麦籽粒胚乳中也存在2种类型的淀粉去分支酶，一种是以极限糊精为特异性底物的极限糊精酶，另一种是以支链淀粉为底物的异淀粉酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失