纯化

In order to exploit the resources ofCrocus sativus and crocin further, molecular biological research of saffron wasstudied in the thesis.

为了研究西红花药用成分西红花甙的资源，更好地利用西红花甙，本论文对西红花进行了分子生物学方面的研究：对西红花酸合成酶(7，8-二加氧酶)基因的克隆，并将其在大肠杆菌中表达，纯化诱导蛋白，制备相应的特异性抗体。

Induction of Defense Responses in Cucumber Plants Cucumis satfaus L.

抗小麦赤霉病抗生素861的提取、纯化及理化性质。

Results:The cytolytic activity of fresh NK cells and purified NK cells from LAK cells was highly increased,but that of CD3AK cells was lowered.The cytotxicity of LAK cells was not affected.

结果：外周血新鲜NK细胞和纯化NK细胞对K562的杀伤率明显提高，CD3AK细胞的杀伤力明显降低，LAK细胞的杀伤无明显变化。

In this research, the isolates of TuMV from Shandong were separated and purified. Their biological, serological, cytopathological and particle morphological properties were studied.

本研究分离纯化了芜菁花叶病毒的山东分离物，对其生物学、血清学、细胞病理学和粒子形态学特征进行了研究。

Cytophaga sp was selected for heparinase production because it was more productive. Three heparinases, named HⅠ, HⅡ and H Ⅲ were purified from the crude cell extract by means of batch DEAE-Cellulose negative adsorption, Hydroxylaptide adsorption and releasing, SP-Sepharose cation exchange chromatography, Source 30S cation exchange chromatography, Poros 20HS cation exchange perfusion chromatography and Sephacryl S200HR gel permeation chromatography.

利用超声破碎、DEAE-纤维素反吸附去杂、羟基磷灰石批处理吸附层析、SP-Sepharose离子交换层析、Source 30S离子交换层析、Porous20HS离子交换灌注层析及Sephacryl S200HR凝胶排阻层析，从噬纤维菌中纯化出三种新的肝素酶。

The technique of refining mucopolysaccharide from Dasyatis akajei cartilage was studied and the content of mucopolysaccharide was measured.

本文探索了利用碱浸提和酶法去蛋白从赤魟软骨中提取分离纯化粘多糖的技术，并对多糖进行了含量测定。

The technique of extracting and refining mucopolysaccharide from Dasyatis akajei cartilage obtained was studied and the content of mucopolysaccharide was measured.

探索利用碱浸提和酶法去蛋白从赤魟软骨中提取分离纯化黏多糖的技术，并对该多糖进行了含量测定。

Ferritin from the liver of a salt water fish, Dasyatis akajei, herein called DALF, was isolated and purified by thermal denaturation, ammonium sulphate fractionation and ammonium reverse extraction, Sephacryl S-300 gel filtration and DEAE- cellulose anion exchange chromatography.

通过热变性处理、硫酸铵分级沉淀、排阻层析和离子交换层析等技术，分离纯化了魟鱼肝铁蛋白Liver ferritin of Dasyatis akaiei。

Methods:Use methods such as take off protein,calcium,colour,deacetylate and purification to prepare chitosan to study deacetylate degree,molecular weight and other characters of chitosan,then compare with infrared spectrum of bio-chemical chitosan on market.

用脱蛋白、除钙、脱色、脱乙酰基、纯化等方法制备壳聚糖，研究其壳聚糖的脱乙酰度、分子量等性质，并与市售生化级壳聚糖的红外光谱进行比较。

objective:with the local lobster shell as the raw material,we inquire into the abstraction and preparation of chitin and chitosan to develop a new raw material resources for chitosan.methods:use methods such as take off protein,calcium,colour,deacetylate and purification to prepare chitosan to study deacetylate degree,molecular weight and other characters of chitosan,then compare with infrared spectrum of bio-chemical chitosan on market.results:the chitin and chitosan obtained from lobster shell are 14.8% and 8.9%, respectively.

目的：以地产龙虾壳为原料，探讨其甲壳素的提取和壳聚糖的制备方法，以开发壳聚糖制备的新原料资源。方法：用脱蛋白、除钙、脱色、脱乙酰基、纯化等方法制备壳聚糖，研究其壳聚糖的脱乙酰度、分子量等性质，并与市售生化级壳聚糖的红外光谱进行比较。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失