纯化

In addition, a kind of copepoda was experimentally cultured, which from the resting eggs separated from the pond mud, then the cultural conditions and biological characters were partly mastered.

分离池塘底泥中桡足类的休眠卵，进行孵化并培养，初步掌握了其中一种桡足类(克氏纺锤水蚤Acartia clausi)的培养条件和部分生物学特性，并进行了纯化培养，为进一步研究病毒传播途径提供充足的实验材料。

Polysaccharide of fruiting body and polysaccharide of mycelium were isolated from Coprinus comatus, and their antiviral activity were researched.

从毛头鬼伞子实体和菌丝体提取多糖，并初步纯化，采用半叶法和叶碟法对抗病毒活性进行测定。

In this paper, modern isolation and analysis methods were used to study on the extraction, purification、characteristics and bioactivities of Coprinus comatus polysaccharides, systematically. The research offers some scientific basis for comprehensive utilization of Coprinus comatus.

本论文以毛头鬼伞子实体为原料，利用现代分离分析手段，对毛头鬼伞粗多糖的提取纯化工艺、理化性质、化学结构以及毛头鬼伞多糖的体外免疫活性进行了较系统的研究。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrin's (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrins (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The optimum extraction process of preparation was carried out with orthogonal test,and the craft of extraction by the single factor experiment, and I suruey the corrigent to choose suitable quantity. This result would be technology parameters for production of Shuanghuanglian Oral Liquid.

通过正交试验优选出双黄连口服液最佳的提取工艺，再通过单因素试验考察纯化工艺，并对矫味剂进行考察、筛选，从而为大生产提供合理可行的技术参数。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

AIM: To study the technological parameters of the purification process of total flavonoids of Crataegus leaves with HPD-600 macroreticular resin.

目的：研究HPD-600大孔树脂纯化山楂叶总黄酮的工艺条件及参数。

Keywords HPD-600macroreticular resin;total flavonoids of Crataegus leaves;separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

HPD-600macroreticular resin ; total flavonoids of Crataegus leaves ; separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失