纯化

ConclusionThe AB-8 resin is suitable for separation and enrichment of chromone glycoside in extract of Yupingfeng.

结论AB8大孔吸附可用于玉屏风提取物中色原酮苷活性成分的分离纯化。

Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

The experiment results showed that the content of chromone glycoside in the extract of Yupingfeng increased from 0.55% to 10.01% with a recovery rate of 87.2% by separation on fixed bed of AB-8 resin.

结果AB8树脂的吸附性能优于其它考察的树脂，经AB8树脂固定床分离纯化后，玉屏风复方提取物中色原酮苷含量从0.55 %提高到10.01%，回收率为87.2%。

ObjectiveTo separate and purify the chromone glycoside from the extract of Yupingfeng with macroporous resin absorption.

目的大孔树脂吸附分离纯化玉屏风复方提取物中色原酮苷成分。

High-speed countercurrent chromatography is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

From 300 mg of the crude extracts, 25.5 mg of baicalein and 36.6 mg of chrysin were obtained with a purity of 99.2% and 100%, respectively, as determined by high performance liquid chromatography.

从300 mg木蝴蝶粗提物中一步分离纯化得到25.5 mg黄芩素和36.6 mg白杨黄素。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

Here, a new method for large-scale preparation of Cinnamomin, a RIP from Cinnamomum camphora was established.

本文对香樟Ⅱ型核糖体失活蛋白Cinnamomin进行了大规模的抽提和纯化。

Ten compounds were isolated from Cirsium henryi (collected from Zhang County, Gansu Province), structures of 7 compounds have been identified.

从采自甘肃漳县的刺苞蓟全草的甲醇提取物中，采用同样方法分离纯化得到10个化合物，鉴定了其中7个化合物的结构，未发现新化合物。

Methods Trizol reagent from clamworm digestive tract epithelial cells extracted methods Total RNA. The mRNA was purified and reverse transcripted into cDNA. The cDNA was linked into plasmid pDNR-Lib after digested by nucleate endonuclease Sfi I to construct cDNA library.

分离沙蚕消化道上皮细胞，使用Trizol试剂提取总RNA，纯化mRNA，用MMLV逆转录成cDNA，用Sfi I核酸内切酶酶切后连接到pDNR-Lib质粒中，构建cDNA文库。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失