纯化

The crude product was treated with Cetyl trimethyl ammonium bromide in order to separate the neutral glycoprotein and the acidic glycoprotein .

本文采用季铵盐沉淀法对粗产品进行部分纯化，分离其中的中性糖蛋白和酸性糖蛋白，最佳分离方案为：pH=7.0，样品浓度为1％，CTAB浓度为3％。

It can be used to purify the extract of Chaenomeles speciosa Nakai.30% and 50% ethanol showed better elution property.

DM-301型大孔吸附树脂表现出较好的综合性能，可以用于纯化资木瓜黄酮；用30%和50%的乙醇作为洗脱剂较好。

Objective: To establish a technical process for the purification of flavonoids from Chaenomeles speciosa Nakai and provide scientific reference for industrial manufacture.

中文摘要：目的：研究大孔吸附树脂分离纯化资木瓜黄酮工艺，为资木瓜黄酮的工业化生产提供依据。

Four experiments were primarily studied the antimicrobial ability and activity of the antimicrobial peptides crude sample from young Chelydra serpentina's intestines,blood,liver,skin,moreover were studied the blood antimicrobial peptides' purification process and their related characteristic.

本文初步研究鳄龟肠道、血液、肝脏和皮肤组织粗提物的抗菌活性，在此基础上进一步研究了鳄龟血液抗菌肽的分离纯化过程及其相关性质。

One hundred and two patients with seasonal asthma allergic to caraway, humulus or chenopodium pollen were divided randomly into 2 groups: observation group and control group and the 2 groups were given purified pollen allergens and crude pollen allergens respectively before the allergic season.

采取随机、对照方法将102例确诊为季节性黄蒿、草、藜草花粉过敏性哮喘患者分为观察组及对照组，应用纯化花粉变应原及粗花粉变应原分别对两组患者进行季节前sit，疗程3 mo。

ABSTRACT Partially purified chlorophyll was used to prepare pheophorbide by chlorophyllase hydrolysis and selective acidic hydrolysis methods.

以初步纯化的叶绿素为原料，利用叶绿素酶水解、选择性酸降解两种方法制备脱镁叶绿酸。

Ion exchanage materials are also used to purify the chlorous acid and chlorine dioxide solutions, without causing degradation of said solutions, to exchange undesirable ions in the chlorous acid and chlorine dioxide solutions with desirable ions, such as stabilizing ions, and to adjust the pH of chlorous acid and chlorine dioxide solutions.

所述离子交换材料也可以用于纯化亚氯酸和二氧化氯溶液，从而在亚氯酸和二氧化氯溶液中用想要的离子如稳定离子交换不想要的离子，并调节亚氯酸和二氧化氯溶液的pH值，而不造成所述溶液的质量降低。

The optimum condition was determined via orthogonal experiments, and the result shows that the technology of bile extraction and purification adopts 10L chromato bar with 3L bile, gel silica as fixed phase, the mixed chloroform and isopropanol as mobile phase, gradient elution with mobile phase (the mobile phase ratio in extraction of cholalic acid and glycocholic acid is 4:1 , and then becomes 3:2 in extraction of glycocholic acid and glycocholic acid), and eluting velocity of 3～5mL/s .

采用正交试验筛选了柱层析最佳条件，以容积为10L的层析柱进行分离纯化，固定相为硅胶，流动相为氯仿和正丁醇混合溶剂，一次装样量3000mL胆汁，流动相采用梯度洗脱，先按4：1比例分离胆酸和甘氨胆酸，然后按3：2比例分离甘氨胆酸和牛磺胆酸，流动相洗脱速度为3～5mL/s。

The study on the extracting small experiment of Choleic acid, Glycocholic acid and Taurocholic acid.The extracting small experiments of Choleic acid, Glycocholic acid and taurocholic acid were studied by adopting deproteinizatio, depigmentation, extraction and column chromatographic fractionation.

提取胆酸、甘氨胆酸、牛磺胆酸的小试工艺研究采用脱蛋白、除色素、萃取、柱层析分离等方法开展了从牛羊混合胆汁中分离、提取和纯化胆酸、甘氨胆酸及牛磺胆酸三种有效成分的小试提取工艺研究。

The immunological competence of the expressed protein was tested by means of Western blotting and enzyme-linked immunosorbent assay, and its biological activity was assayed by chicken chorioallantoic membrane and Matrigel angiogenesis assay.

酶联免疫吸附和Western blot技术检测纯化的VEGF165蛋白免疫学活性，鸡胚尿囊绒毛膜实验和matrigel血管形成实验检测其生物学活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失