纯化

This text is the first one to report the preliminary test for the chemical components in the leaves of Carya cathayensis and C.

首次报道山核桃叶和美国山核桃叶化学成分预试验，有利于对其有效成分的提取、分离、纯化研究，为今后深入研究与开发利用该资源提供一定的研究基础。

Ion-exchange chromatography with DE-52 was used for the purification of the antigens. The purified products were characterized by Western-blotting. Result For the pollen extract of Caryota ochlandra, 30 protein bands were detected in SDS-PAGE.

收集过敏病人血清，采用免疫印迹(Western-blotting)法鉴定其变应原成分，通过离子交换层析对花粉变应原进行初步纯化和免疫印迹鉴定。

It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达，SDSPAGE鉴定表达产物，亲和层析纯化蛋白，酶联免疫吸附实验方法测定整合酶的生物学活性。

The physical and chemical properties of the purified MPH were studied. In the crude extracts, the specific activities of MPH for methyl parathion and fenitrothion were higher than those of OPH from F.sp, while the catalytical activity for parathion was lower than that of OPH.

我们根据Pseudomonas sp WBC-3的生长特性及产酶情况，以阳离子交换层析纯化胞内粗蛋白中，使酶的比活提高了49.1倍，得到了均一的目的蛋白MPH，SDS-PAGE验证为单一的条带，。

Coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions, the sensitivity test, the specificity test, repeatability test and sample simulation test, the indirect ELISA method was established, which is a broad-spectrum, rapid and specific to detect various of food-poisoning Vibrio simultaneously.

融合毒素蛋白包涵体经纯化，免疫豚鼠制备血清抗体，确定间接ELISA的最佳工作条件，并进行敏感性、特异性、重复性及模拟样品检测试验，建立了食物中毒性弧菌广谱、快速、特异的同步免疫学检测方法。

Objective To study the isolation and purification condition of pristimerin from Celastrus orbiculatus Thunb.

目的 对南蛇藤中扁蒴藤素提取纯化工艺进行研究。

The purification-pretreatment technique of 8 kinds of solid phase extracting column stuffing (silica gel, acid silica gel, Florisil, siliceous magnesium sorbent, Al2O3, celite 545, acid celite, Al2O3+silica gel) for the prepared polluted soil containing 13 kinds of organochlorine pesticides (α-HCH,β-HCH,γ-HCH,δ-HCH, o,p'-DDT, o,p'-DDE, p,p'-DDT, p,p'-DDD, p,p'-DDE, a-endosulfan, dieldrin, endrin, hexachlorobenzene) was screened prior.

用製备的8种固相萃取柱填料（硅胶，酸化硅胶，氟罗里硅土，硅镁型载体，Al2O3，硅藻土载体545，酸性硅藻土，Al2O3+硅胶）对含有13种有机氯农药的污染土壤的纯化预处理技术进行了优化筛选。

Because a very low abundance of MSCs is present in bone marrow (approximately one Per 10〓-10〓 nucleated celled), ex vivo expansion produces a significant increase in the number of MSCs that can be very important.

然而，人体内骨髓中MSC的含量极其稀少，每1×〓-1×〓个单个核细胞中大约有1个MSC，组织工程中对种子细胞的数量要求巨大，如此微量的MSC难以满足组织工程的需要，因此体外纯化和扩增MSC就显得尤为重要。

A protein, designated as cerebrin 30 (Mr. 30, 000), has been isolated and purified from human cerebrospinal fluids obtained from patients with schizophrenia disorders. The methods applied are C8 reversed-phase HPLC, SDS-PAGE in conjunction with HPEC. The N-terminal amino acid sequence of cerebrin 30 (〓-APEAQVSVQPLFXQ) was determined. Specific polyclonal antibody against cerebrin 30 was also prepared.

近来，国外研究人员应用C8反相HPLC、SDS-PAGE以及HPEC等方法从精神分裂症患者脑脊液中纯化了一种蛋白质，定名为Cerebrin 30（Mr.30，000），测得了其N-末端的十几个氨基酸序列，并制备了这一蛋白质的特异性多克隆抗体。

Polysialic acid could form complex with cetyl pidinium chloride specifically and was thus used for the separation of PSA from E.

利用氯代十六烷基吡啶对聚唾液酸有特异性的络合效应来进一步分离纯化聚唾液酸。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失