纯化

The extraction andpurification technology of Agaricus blazei Murill polysaccharide was studied, the optionaltechnology were as follows.

主要对姬松茸多糖的提取及纯化技术进行了研究，确定一条多糖提取及纯化的最佳工艺。

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

The substance with a molecular weight of 6.28kDa has very strong cation character determined by the electrophoresis of diaphragm wafer of fiber of acetic acid. The substance with antibacteria action obtained from forest frog is made up of alanine,aminoacetic acid,leucine,Isoleucine,Proline,aminoglutaric acid,threonine,Serine,lysine.

纯化林蛙皮肤中的抗菌活性物质经醋酸纤维薄膜电泳，表明其具有较强的阳离子特征；经氨基酸分析，表明林蛙皮肤抗菌活性物质是由丙氨酸、甘氨酸、亮氨酸、异亮氨酸、脯氨酸、中国林蛙皮肤抗菌肽的分离纯化和鉴定一摘要谷氨酸、苏氨酸、丝氨酸、赖氨酸组成。

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白，再经割胶回收法得到进一步纯化的重链片段，分子量约为75kDa，免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清，为ELISA和Western blotting检测技术奠定了基础。

The applied extension is expanded and the economic value of saponins f rom Aralia elata Seem is enhanced.

本论文以辽宁本溪县产刺嫩芽为原料，系统研究了刺嫩芽皂甙的提取纯化方法与条件、刺嫩芽皂甙基本性质、β-葡萄糖苷酶处理对皂甙影响及酶处理后刺嫩芽皂甙生理活性的提高程度，确定出了刺嫩芽皂甙的提取纯化工艺和降低毒性提高活性的方法，扩大了刺嫩芽皂甙的应用范围，提高了刺嫩芽的经济价值。

The expression of CSE1L gene was identified using these aRNA by sqRT-PCR.

结合RNA保护、显微切割及线性扩增技术，获得纯化鼻咽组织中反义RNA（antisense RNA，aRNA），采用半定量逆转录聚合酶链式反应（semi-quantitative reverse transcriptionpolymerase chain reaction，sqRT-PCR）研究从全基因组表达谱中筛选的候选癌基因-CSE1L在两种不同纯化鼻咽组织aRNA中的表达。

To exploit highly bioactive natural antifungal medicaments from starfish, water-soluble starfish glycosides from Asterias rollestoni were isolated and purified by macroporous resin column and silica gel column chromatography, and their antifungal bioactivities were also examined.

为了分离纯化出具有强抗真菌活性的水溶性海星皂苷，以罗氏海盘车腕为实验材料，利用水抽提方法获得水溶性海星皂苷粗品，依次利用大孔树脂和硅胶柱层析对水溶性海星皂苷进行了纯化，并对其抗真菌活性进行了测定。

Using awater purification cartridge beyond its expiration date or failing to clean the system on aregular basis can lead to water with higher resistivity as well as microbial contamination.

设备所用的纯化柱填料过期或没有按照程序来清理纯化柱，可能会导致水的电阻率升高或被细菌污染。

In this thesis betulin was purified by recrystallization with chloroform and methanol mixture, and orthogonal design had been taken to optimize the conditions of recrystallization including the volume and ratio of chloroform and methanol mixtures.

在白桦酯醇的纯化方面目前比较成熟的方法是氯仿+甲醇混合溶剂重结晶来纯化白桦酯醇，本文通过正交设计，得到最优化的溶剂比例和用量。

For purification of McAb,the results suggested that ion exchange chromatography method can be applied for analysis and caprylic acid method for a very large volume of ascitic fluid.

单抗纯化结果显示离子交换层析法适用于科研分析，而辛酸沉淀法则适用于大量腹水中单抗的纯化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失