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White Spot Bacilliform Virus; Microarray; Expression and purification of protein; Immune electron microscopy; Protein-DNA binding interaction

对虾白斑杆状病毒; Microarray;蛋白表达与纯化;免疫电镜技术;蛋白质-DNA结合

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles.

利用大肠杆菌表达系统可溶性表达人乳头瘤病毒18型(HPV18)L1蛋白,经过纯化和重组装过程获得HPV18病毒样颗粒,研究其免疫原性和诱发中和抗体生成的水平。

The extraction and purification of Acetobacter Z127 ADH. Four methods were employed to isolate ADH from Acetobacter Z127 just to find bacteriolytic solution could break cell efficiently and maintain ADH activity simultaneously and thus it was suitable for the extraction of Acetobacter Z127 ADH.

通过实验对模型的检验,表明实验建立的细胞生长动力学模型和乙醇消耗动力学模型可以反映Acetobacter Z127的生长、消耗反应规律。3Acetobacter Z127中ADH的提取纯化

The triterpenoid constituents in the extract of Banaba leaves were separated by resinadsorption.

采用树脂吸附法对大叶紫薇叶中的三萜化合物进行了纯化

The molecular structures of APSP were identified. BAW was selected as the eluent of GC through TLC. After separation and purification, two anthocyanins were obtained.

本文对APSP组分进行了研究,首先通过薄层层析实验选定以BAW体系为柱层析洗脱剂,然后对APSP进行分离纯化,得到两种相对含量较高的组分Ⅰ和Ⅱ,并对组分Ⅰ和Ⅱ进行了一系列结构鉴定实验。

From the ester n, the intermediate Ⅲwas produced by the Sonogashira coupling reaction with terminal alkynes under the co-catalysts of Pd2 and Cul. These final products of alkynyl benzamide derivatives IV were obtained by the cleavage of support with primary or second amines.

液相合成兼容了固相合成可快速简便地对产物进行分离纯化以及溶液相合成可在均相条件下进行反应和用常规手段对反应中间体进行分析检测的优点,用聚乙二醇4000作为可溶性聚合物支持体,通过醚键将[1,3,5]氯三嗪连接在PEG上制备成PEG支持的新型的可溶性聚合物试剂I。

Seventy two percent of the cells stained with benzidine. Poly〓 RNA was isolated from both the cells directly from separation and the cells cultured with EPO for 36 hours.

将未培养的FVA细胞(0时)和经36小时培养的FVA细胞(36时)分别进行RNA提取,并纯化其mRNA,逆转录成cDNA(cDNA-0和cDNA-36)。

C. In addition, they introduced a fluorosubstituted benzylic ester (CH 2 C 6 H 4 CH 2 CH 2 C 8 F 17 ) as a protecting group in solution-phase peptide synthesis to facilitate the purification of intermediates by fluorous-solid-phase extraction.

丙此外,他们推出了fluorosubstituted苄酯(丙6小时2楚楚242丙的CH8六17)为保护群液相肽合成纯化中间体的便利氟-固相萃取。

In addition a simple process was also developed to obtain purified betulin using chloroform / NaOH two-phase solvent extraction.

另外还建立了一种在碱性条件下用氯仿/水溶液混合溶剂来一步纯化白桦树皮中的白桦酯醇的方法。

The invention relates to a preparation and purification method of bicaudal gemellus quaternary ammonium salt type cationic surfactant.

本发明涉及一种双尾双子季铵盐型阳离子表面活性剂的制备及纯化方法。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。