纯化

The optimum process was as follows : solvent is 0.3% ammonia solution , ratio of solvent to solid ratio is 10:1 , vacuum-cavitated time is 15 minutes , GA extraction yeild is 2.0%, the crude extract is 30.3%, and in which the content of GA is 6.6%.

采用负压空化方法对超临界萃取后固形物中甘草酸的提取工艺进行了研究，并研究了甘草酸纯化的工艺，通过影响因素试验和正交试验得出最佳工艺条件为：0.3%的稀氨水为溶剂，液固比为10：1，负压空化提取15min，甘草酸提取率为2.0%，含量6.6%。

OBJECTIVE:To obtain high purified and cheap human copper/zine superoxide dismutase with genetic engineering and affinity chromatography.

目的：用基因工程的方法获得人铜锌超氧化物歧化酶的高产菌，通过发酵培养，最后纯化获得大量廉价的高纯度的hCu，Zn－SOD。

Because the physical and chemical properties of 5'-Cytidine triphosphate,5'-Cytidine disphosphate and 5'-Cytidine monphosphate are very similar, and thereare many impurities in the solution of zymolysis, the separation of down stream isgreatly difficult.

由于三种磷酸胞苷的理化性质很相近，而且三磷酸胞苷酶转化液中各种杂质含量较多，导致下游分离纯化很困难。

The exact of petroleum ether was the acaricidal active site of Eungenia caryophyllata. 3.Isolation, purification and identification of compounds with acaricidal activity in Eugenia Caryphyllata: The exact of petroleum ether was separated into 6 parts by silica gel column chromatography with gradually increasing polarity of petroleum ether: ethyl acetate. The part C was demonstrated to be the most effective part by acaricidal activity test in vitro,and the time for it to kill all experimental mites was about 20 min.

丁香杀螨活性成分的追踪分离、纯化与结构鉴定：采用硅胶柱层析法，以石油醚：乙酸乙酯溶剂系统梯度洗脱，对丁香石油醚提取物进行分离，得到A~F 等6 段混合物，离体杀螨试验发现，C 段混合物具有突出的杀螨活性，50%浓度平均15min可杀死全部供试螨虫。

All the compounds were isolated and identified from the bituminous coals for the first time, bis(2-ethylheptyl) terephthalate and 18-ethyl-17-(hexan-2\'-yl) docosanoic acid are new compounds.Further more, a Colourless Oil Liquid, a white acerate crystals containing some alkanes, a white tabular crystals consisting of some fatty acid esters and a white powder crystals in which some fatty acids exist were isolated and analysed by GC/MS for the first time.Through this research, a study method of"classification extract of bituminous coal- separation with silica-gel and gelatin column chromatographies- identification of the isolated compounds by FTIR, GC/MS, LC/MS, 1H NMR and 13C NMR analyses"was established and proved to be a practical approach toward separable and non-destructive identification of some organic compounds in bituminous coals for coal chemistry research.

本研究建立了&原煤分级萃取-硅胶与凝胶柱层析分离与纯化-GC/MS或LC/MS检测-FTIR、MS、1H NMR和13C NMR波谱解析&这一煤中有机化学成分的非破坏性分离与分析的研究方法，并验证该研究方法有效可行。

Compounds were extracted by 90% ethanol-petroleum ether and acetoacetate, then purified by silica gel column chromatography.

采用硅胶柱层析进行分离纯化，根据理化性质和波谱学数据进行结构鉴定。

Kombucha was fermented with pure strains of yeast Y1, lactobacillus R3 and acetobacter C5, and the technical conditions of fermentation were optimized.

采用自行分离纯化的酵母菌Y1、乳酸菌R3、醋酸菌C5进行纯种混合发酵生产红茶菌饮料，并对发酵工艺条件进行优化。

Their structures were detailedly identified on the basis of chemical methods (sugar composition analysis, glycosyl residue linkage analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, acetolysis, Congo Red experiment, etc.), spectral technologies 1D, 2D NMR, ESI-MS, IR, etc.

通过各种化学方法（糖组成分析，糖残基连接方式分析，部分酸水解，高碘酸氧化，Smith降解，乙酰解，刚果红实验等）、光谱方法（1D、2DNMR，ESI-MS，GC-MS，IR等）和物理化学方法（分子量分析，粘度分析，旋光度分析等）对各纯化多糖的化学结构及糖链构象进行了鉴定。

In this study, a strain of bacteria capable of producing Phenylalanine dehydrogenase was isolated and was identified as Achromobacter sp. The condition for the enzymatic fermentation, purification and characteristics of the enzyme were carried out in this work.

本研究从土壤中分离筛选到一株产苯丙氨酸脱氢酶的菌株X5-3，对其进行鉴定，并进一步研究了该菌株的产酶发酵条件、酶的初步纯化及该酶的性质。

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法，首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白，并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失