纯化

The stimulations of wheat tonoplast H +\|ATPase by both Mg 2+ and Ca 2+ were measured on the base of purification of the enzyme.

纯化小麦液泡膜H+-ATPase，测定了纯化的小麦液泡膜H+-ATPase 受Mg2+和Ca2+双重激活的特性及两种激活状态下酶的特性。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

It was further purified by ultracentrifuge and repeated precipitation and purification with ethanol to obtain the required polysaccharide components, in which MEP\|SP of molecular of 10 000～100 000 was purified and separated by...

对其中分子量为 10 0 0 0～ 10 0 0 0 0的多糖，采用DEAE 纤维素离子交换柱，以不同浓度NaCl为洗脱液，进行纯化分离，再经SepharoseCL 6B纯化得到 2种水溶性多糖MEP SP2和MEP SP3。

The distributions of aminoacylase and alkaline phosphatase had been studied in the common seaweeds in Dalian area, and the Ulva pertusa Kjellm had been chosen as the target for isolation and purification. These two enzymes had been purified for a single band in the SDS-PAGE and their basic characteristics had been studied, too.

研究了氨基酰化酶和碱性磷酸酶在大连地区常见海藻中的分布状况，从中筛选出孔石莼作为纯化研究对象，并从孔石莼中分离纯化出了这两种酶，纯度达到了聚丙烯酰胺凝胶电泳一条带。

Extraction, purification process and stability of the pigment of chestnut shells were explored. The results showed that this pigment dissolved easily in alkaline solution, but undissolved in noapolar solvent.

该文以板栗壳为材料，研究板栗壳色素的提取、纯化工艺及纯化色素的稳定性，为板栗壳色素的推广应用提供理论基础和实验数据。

Yes, because, basically, you're bringing in the active ingredient in an unpurified form and subjecting it to the purification.

因为，你们基本上购买了未纯化的活性物质将它纯化。

Unpurified protein. 2:Extract of cells infected with vaccinia virus 3:Extract of cells un-infected with virus. 4:Purified protein

层析纯化前的粗提物； 2：野型病毒感染的细胞粗提物；3：未经感染的细胞粗提物； 4：经纯化的蛋白

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

This second exhibit can exhibit times Warton 8 inches, wait for whole set product 4 inches with family expenses film, ultralow pressure, low pressure, fight pollute and fight the international such as oxidation banner technology and come to a company a few years be in briny desalt, sewage purification, medium water is answered use, drinking water industrial water is rarefied, rarefied and the arrogant person outstanding achievement that condenses the field place such as depart to obtain and applied technology.

本次展会展出了时代沃顿8寸、4寸和家用膜等全系列产品，超低压、低压、抗污染和抗氧化等国际领先技术以及几年来公司在海水淡化、污水净化、中水回用、饮用水纯化、工业水纯化以及浓缩分离等领域所取得的骄人业绩和应用技术成果。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失