纯化

Roots as material, a tight tonoplast preparation with high purity was isolated by differential centrifugation and sucrose density gradients centrifugation.

根为材料，采用差速离心和蔗糖密度梯度离心法分离纯化液泡膜。

Objective:To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin.

目的：优选大孔吸附树脂富集纯化牡丹皮中丹皮总苷的最佳工艺参数。

Purity,trenchancy,accuracy and beautification." Little " also thinks of the aesthetic of ages as an important measure of poster design.

"少"的真正意思就是招贴设计应突出主题，即其所反映出的主题内容要单纯化、鲜明化、准确化以及唯美化

For purification of nitrogen trifluoride(NF3) which is used as a plasma etchant and cleaning gas for chemical vapor deposition chambers, a few purification methods and equipments may be chosen according to difference in boiling points and physico-chemical properties of NF3 and its impurities.

三氟化氮作为等离子蚀刻剂和化学气相沉积室的清洗气体，可根据粗三氟化氮气体中各组分的沸点及物理、化学性质的差异选择不同的纯化工艺及设备。

Objective To screen the mimic antigen epitopes of the triose phosphate isomerase of Schistosoma japonicum Chinese strain and investigate their immunogenicity.

用纯化的抗重组SjC TPI抗体筛选 12肽库，获取SjC TPI的模拟抗原表位，并研究其抗原特性。

Triose phosphate isomerase was prepared and purified from chicken breast muscle. The equilibrium unfolding of TIM induced by guanidine hydrochloride was investigated by following the changes in intrinsic fluorescence, circular dichroism and second-derivative spectroscopy.

从鸡胸肌中纯化出磷酸丙糖异构酶(triose phosphate isomerase， TIM)，通过蛋白质内源荧光，圆二色性，紫外吸收二阶导数光谱等多种研究溶液构象的方法，对TIM被盐酸胍和热变性过程进行了详细的研究。

We studied the effects of different organic solvents and inhibitors on the decolorization of recombinant triphenylmethane dyes decolorization enzyme, expressed extracellularly from Pichia pastoris.

本文在利用毕赤酵母成功分泌表达高活性三苯基甲烷类染料降解酶TpmD的基础上，研究不同有机溶剂和常见酶抑制剂对纯化后的重组脱色酶TpmD活性的影响。

The impurities generating mechanism is explored and corresponding technical plan is provided for determining and diminishing the impurities including diphenyl methane, triphenylmethane, triphenylcarbinol, 4-methy benzophenone, 4-chloro benzophenone and etc. in order to produce high purity benzophenone.

利用化学技术、循环经济和产业生态学原理结合本生产工艺过程的特点确立包括碳循环、苯循环、工艺水循环、氯的回收循环等闭合循环技术组合，设计了光气即产即用技术、倾析分离技术、能量多级利用技术、蒸发—冷凝联用和多热源组合集成技术、二苯甲酮纯化技术等实用方案，实现经济地生产清洁的高纯度产品和生产过程废物零排放。

Hybridomas are prepared by the fusion of mouse myeloma cells,cell line P_3-X63-Ag8.653,and spleen cells from BALB/C mice immunized with partially clarified and concentration sap from Citrus tristeza virus infected citron.

以感染枸橼的柑桔速衰病毒(Citrustristeza virus，CTV)的部分纯化制备物和浓缩的感染组织粗浸出液免疫BALB/C小白鼠后获得的脾脏细胞，与鼠骨髓瘤细胞株P3-X63-Ag8.653融合制备了杂交瘤细胞。

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失