纯化

Objective To clone and express Taenia saginata asiatica's Spef1like gene,and purify its protein.

目的 扩增、克隆和表达亚洲牛带绦虫Spef1Like基因全长cDNA，并进行表达产物的纯化。

Taenia saginata asiatica's Spef1like gene was successfully cloned and its expression and fusion protein was purified,which provides favorite conditions for further studies of the gene.

成功克隆了亚洲牛带绦虫Spef1Like基因，并表达和纯化了Spef1Like蛋白，为研究Spef1Like的功能及其疫苗等研究提供了基础依据。

We first use magnetic nanometer particles to purify the GRP, then take count of the living cells which induced by GRP.

实验用磁性纳米粒子吸附法纯化水提物中的蛋白成分，通过活细胞计数法确定以连有纳米粒子的GRP为实验药物。

With rarefied the albumen after is vaccine of oily emulsion of immune former preparation immune egg chicken, the result makes clear, CCK albumen can cause immune reaction inside gallinaceous body, tenability of antibody of the CCK in serum of chicken of the egg after strengthening immunity and yolk is in higher level.

以纯化后的蛋白为免疫原制备油乳剂疫苗免疫蛋鸡，结果表明，CCK蛋白可在鸡体内引起免疫反应，加强免疫后蛋鸡血清和卵黄中CCK抗体可维持在较高水平。

Porphyridium cruentum were treated with some antibiotics which were used as genetic selected marks in genetic engineering. The results showed that the algae cells could resist high concentration of Penicillin,Streptomycin,Gentamicin and Kanamycin, without any influence at the dose below 100 u/mL of which can kill bacterium adhere to the algae cells . These antibiotics can be used to repress bacterium in purifying algae strain.Porphyridium cruentum is high sensitive to Erythromycin,Terramycin and Ofloxacin....

研究了基因工程中常用抗生素标记对紫球藻的生物学效应，结果表明紫球藻对青霉素、链霉素、庆大霉素以及卡那霉素较不敏感，剂量在100u/mL以下对其生长无显著影响，但能抑制或杀灭伴生的杂菌，因此这些抗生素可用于藻种纯化过程中抑菌；紫球藻对红霉素、氧氟沙星和土霉素非常敏感，低质量浓度就能杀死藻细胞，可用作基因工程或遗传育种的抗药性选择标记；对四环素和氯霉素较为敏感，明显抑制细胞生长，并呈浓度梯度效应。

The results of SDS-PAGE showed that the expression products were about 31KD and reached their expression peaks about 2 hours after inducing and the expression quatity of fusion protein had 22.7% ratio of total thalline protein, while the E.

提取并纯化pET-PIL-8的融合表达蛋白并用改进的琼脂扩散法检测其生物学活性，通过检验分析表明所获得的重组猪IL-8具有趋化猪外周血淋巴细胞的作用，具有较强的生物学活性。

Thermolysis of DM-DMI bis-carbamate was carried out in 3wt % solution of diphenyl ether at 254℃ for 5-6 hours, and the yield of DM-MDI is 61％ after purification.

而在第二步骤的热裂解反应中，在3wt ％二苯醚溶液中，控制反应温度在254℃，经反应5-6小时即得到DM-MDI，经浓缩及纯化后主产物之产率为61％。

An investigation on the acquired thermotolerance of recombinant JP2 ligase was done by applying a chaperonin known as TF55 in thermophile on JP2 ligase reaction.

还分离纯化了JP2的分子伴侣－TF55，并将其应用于增加JP2连接酶的热稳定性研究。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

However, the titration and protein concentration of IBDV in vero cells was higher than that in CEF cells.

因此选用异源的咖细胞大量增殖IBDV，并采用不同的方法进行纯化比较。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失