纯化

Methods: Rat MSCs was isolated and purified in vitro, labelled by Hoechst33342, stereotaxis imbed into CA4 of rat hippocampus. To kill rat and recipe brain after 1,2,4 weeks to be made to frozen section, amd to observe Hoechst33342-marked cells under fluorescence microscope.

分离大鼠MSCs体外培养、纯化，Hoechst33342标记后立体定向植入大鼠海马CA4区，1、2、4周后处死大鼠取脑，制作冷冻切片；用荧光显微镜观察Hoechst3342阳性细胞。

The purification method of the sterol ester was also studied.

同时研究了甾醇酯的分离纯化方法。

The structure and morphology of CNTs which have been purified and not yet, were characterized by TEM, SEM, XRD, Raman, TG-DTA, BET, IR. The results indicated that when the stokehole reaction temperature was 1170℃,the decompose temperature of Ferrocene was 150℃,he volatilize temperature of Benzene was 50 ℃, hydric flow rate was 270ml/min, the concentration of thiophene was l% and the reaction time was 20 minutes, the CNTs with single-walled and multi-walled were prepared.

结果表明：炉膛反应温度1170℃、二茂铁的分解温度150℃、苯的挥发温度50℃、氢气的流量270ml／min、噻吩体积分数1.00／100ml苯、反应时间20min时，可以得到碳纳米管，既有单壁管又有多壁管，单壁管的内径分布在0.88～1.15nm之间，多壁管的直径分布在40～120nm之间；纯化后的碳纳米管比表面积增加，管壁光滑，而且产生了大量的含氧官能团，有利于在水溶液中的分散。

The structure and morphology of CNTs which have been purified and not yet, were characterized by TEM, SEM, XRD, Raman, TG-DTA, BET, IR. The results indicated that when the stokehole reaction temperature was 1170℃,the decompose temperature of Ferrocene was 150℃,he volatilize temperature of Benzene was 50 ℃, hydric flow rate was 270ml/min, the concentration of thiophene was 1% and the reaction time was 20 minutes, the CNTs with single-walled and multi-walled were prepared. The diameters of the single-walled CNTs are in the range of 0.08~1.15nm, and that of the multi-walled CNTs are in the range of 40~120nm.

结果表明：炉膛反应温度1170℃、二茂铁的分解温度150℃、苯的挥发温度50℃、氢气的流量270ml/min、吩体积分数1.00/100ml苯、反应时间20min时，可以得到碳纳米管，既有单壁管又有多壁管，单壁管的内径分布在0.881.15nm之间，多壁管的直径分布在40120nm之间；纯化后的碳纳米管比表面积增加，管壁光滑，而且产生了大量的含氧官能团，有利于在水溶液中的分散。

Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically.

采用涂布法、划线分离法以及形态学和生理生化特性分析，对湛江湖光岩微生物进行了分离纯化及部分性质研究；同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。

A low-molecular xylanase produced by Streptomyces sp. Z18 was purified and characterized in this study.

研究了链霉菌Z18中一种低分子质量木聚糖酶的纯化和性质。

The isolation and purification of β-NGF form mice submaxillary gland werecarried out by using CM-FF ion exchanger.

本文采用国产阳离子交换树脂CM－FF，两步柱层析从小鼠颌下腺中分离、纯化神经生长因子，并与Whatman公司的CM-C（CM32）离子交换树脂进行了比较。

Methods: The NGF purified from mouse submaxillary glands was packed with liposome and prepared into freeze dying agent.

将纯化得到的小鼠颌下腺的NGF用脂质体包裹后分别制成冻干剂型。

Objective: To establish a consummate system of extraction,purification and analysis of type Ⅱ Collagen that use as chondrocytes culture substratum.

目的：建立一套完善的提取、纯化、检测Ⅱ型胶原的生产系统，并以此产品应用在软骨细胞培养上。

This practice–based on Sulu Moro interpretations of jihad –consisted of elaborate dedication and purification rites conducted with family and religious authorities.

这个实践基础上对苏鲁摩洛诠释圣战组织-包括详细的献身精神和纯化仪轨进行，与家人和宗教当局。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失