纯化

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

The serum samples were detected by Indirect ELISA and Indirect hemagglutination test as reference method to assess the value of the recombinant protein in serodiagnosis.

结论1、大量表达并纯化了重组蛋白SjRPS4，并证实其具有良好的免疫反应性，能与日本血吸虫病阳性血清特异性结合，可应用于日本血吸虫感染血清学诊断。2、成功建立了以重组蛋白SjRPS4为包被抗原的间接ELISA法，在日本血吸虫病的免疫诊断中具有良好的应用价值。

The various components of the system will be described in the following order(1)nutrient broth,(2)serum or serous fluid,(3)strain of RPneumococcus, and (4)extraction,purification,and chemical nature of the transforming principle.

意思大致是：该系统的各个组成部分，我会按以下列顺序进行描述：（1）营养液，（2）血清或浆液性液体，（3） R 的肺炎球菌菌株，（4）提取，纯化和化学性质的转化条件。

To determine the structures of two sesquiterpene compounds isolated from Commiphora opobalsamum, and to investigate its anti-proliferation effect on tumor cell growth.

目的从没药提取物中分离纯化倍半萜单体成分，并探讨其抑制肿瘤细胞增殖的生物活性。

A nematicidal sesquiterpene was isolated from the ethyl acetate extract of Michelia hedyosperma Law according to bioassay-guided fractionation.

根据活性追踪分离，从香子含笑叶的乙酸乙酯提取物中分离纯化到一个杀线虫的倍半萜。

The inocula of oocysts were collected from the droppings by purifying with Sheather's sugar flotation method. Two days-old chickens were inoculated by purified inocula of 10,000 oocysts, and vaccinated with one dose of MDV (CVI988 strain) simultaneously.

籍由Sheather's sugar flotation的方法所纯化隐孢子虫的成熟卵囊，经品接种每只10，000卵囊於1日龄之小鸡，并同时皮下接种1剂量的MD疫苗(CV1988株)，於接种后第7天和第12天抽血分离血清，与得自MD病鸡羽毛囊的MD病毒沉降抗原作用，经由琼脂沉？

Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测，提示传代的细胞均一性较好，表面抗原CD34表达阴性，CD44表达阳性的细胞均在95%以上，说明得到的是比较纯化的MSCs。

The separation and purification of pristinamycins from the fermentation broth by the method of silica gel column chromatography was developed.

建立了对发酵液中的普那霉素进行分离纯化的硅胶柱层析方法。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

The results suggest that our method for obtaining fraction antigens is simple and reliable by purifying AWAm, AWAf and SEA with DE22 cellulose.

AWAm，AWAf，SEA经DE22纤维素层析纯化获得组分抗原，方法简单、可靠。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失