纯化

Because it is a hard work to refold the denatured inclusion proteins correctly, especially for those which contain several disulfide bonds. the results?repeatability of some activities is not very perfect.

纯化包涵体后，将产物变性成可溶形式，然后用Ni-NTA对表达产物纯化，SDS-PAGE检测为一条带，包涵体的复性是一项非常困难的工作，尤其是对含有多对二硫键的蛋白质，因此复性后冻干，测它的某些活性时，结果的重复性不太理想。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

There was no loss of biological activity of LL-EPO after labelling as determined by reticulocyte counting method.

离心超滤法分离纯化标记蛋白，与通常应用的凝胶过滤纯化法进行了比较。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Isolation and purification of dihydroagarofuran sesquiterpene in Celastrus angulatus was preformed with normal-phase and reserve-phase high performance liquid chromatography and detected with TLC and HPLC.

为了优化苦皮藤中二氢沉香呋喃倍半萜类化合物分离纯化的方法，应用正相及反相高压制备柱层析相结合的方法进行分离纯化，以TLC及HPLC确定制备流动相条件并跟踪检测其分离效果。

According to breeding objective and the study on characteristics of wild southernwood resources, the plant types of southernwood were directionally screened by derived system method and group mixed method, the selected population were purified and their characters were identified, the variety comparative tests in muti-sites for many years were conducted for the purified types that passed identification.

方法］根据育种目标和对青蒿野生资源特征特性的研究，采用派生系统法和集团混合法对青蒿植株类型进行定向筛选，对中选群体进行纯化和性状鉴定，对通过鉴定的纯化类型进行多年多点品种比较试验。

The result of ISH revealed that the mTSARG3 gene is expressed mainly in spermatocyte.

将PQE30／mTSARG3原核表达产物进行纯化，SDS—PAGE显示纯化后产物是分子量为36kD的蛋白。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Isolation purification of NRCs:The NRCs were collected from the heart of neonatal rats,they were digested with steapsin and different speed of adhesive-screeing .

NRCs的分离、纯化：取出生1天的SD乳鼠，用胰酶消化和差速贴壁方法分离、纯化NRCs。

Until now, studies about polysaccharide from tartary buckwheat are few in china. This paper was mainly about the method of purification of the polysaccharide from tartary buckwheat, and it also analyzed the physiological functions of the purified fraction of the crude polysaccharide.

目前国内对苦荞多糖的研究较少且不够深入，本论文以苦荞麦麸为原料进行多糖的分离纯化，并对粗多糖的纯化组分进行分析以研究其生理功能。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失