纯化

Methods Proteins existed in adult Schistosoma japonicum membrane were extracted by Triton X-100 and purified through reverse-phase high performance liquid chromatography,and the main protein peaks were then collected separately. 125 I-LDL of human plasma as the ligand, through the radioautography and radioligand binding assay, the protein which can bind human serum 125 I-LDL specifically was identified.

自人工感染家兔的肠系膜下静脉取日本血吸虫成虫，用非离子去污剂TritonX 10 0抽提出日本血吸虫成虫表膜蛋白，反相高效液相色谱进行分离纯化后，收集各主要分离蛋白峰，以12 5I标记的人血浆低密度脂蛋白作为放射性配体，经放射性自显影及放射性受体配体结合分析鉴定能与人血浆12 5I LDL特异结合的蛋白。

A method of the preparation of standard radioactive solution of ~(241)Am isdeveloped by radiochemical separation and puritication.

本文系统地研究了~(241)Am的分离、纯化、溶液组成及其稳定性、制源技术和放射性活度绝对测量等问题。

Here in the bottom of their test tube lay the world's first partially purified sample of radium.

就在他们的试管中，放着世界上最早的部分纯化的镭的样品。

But it showed intact function to rebind to PSⅡ membrane and recover its oxygen-evolving activity.

纯化的野生型表达蛋白在结构上和天然蛋白有所不同，但却保持了完整的结合PSⅡ膜能力及恢复放氧的功能。

Result:After many repetatur experiments in different conditions,high express and purification from recombination were selected.

结果：经过多种条件的反复试验，实现了重组蛋白的高效表达和纯化。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

This shows that the technology with the macroreticular resinous adsorbent is a key to realize the modernization of CMM.

由此可见，大孔吸附树脂分离纯化中药提取液是实现中药现代化的关键技术。

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC，5％CO。培养箱中培养1－2小时后，弃培养液：lxPBS清洗数次；0.25％胰酶－EOTA 37℃消化5分钟，加入含川％FBS的a－MEM，经小25tim过滤网过滤；IX PBS清洗末过滤细胞，即得到纯化的破骨样细胞，并进行细胞形态学和分于生物学鉴定。

Results The isolated epidermal stein cells of rhesus monkey were near 90%.

纯化后的皮肤干细胞比例接近90%。

Objective:To study the extracting conditions and purification process parameters of Rheum.

目的：研究大黄提取纯化的工艺条件及参数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失