纯化

The chemical constituents was separated and identified by physiochemical and spectroscopic analysis.

方法用柱色谱法进行分离纯化，通过理化性质和波谱数据分析鉴定其化学结构。

The extracting means and applications of extractions that come from rice bran are summarized,the extractions include rice bran oil,rice bran protein, rice bran polysaccharide ,rice bran phytin et al.

米糠多糖是指从稻糠中提取纯化的一种以α-1，6-糖苷键为主要结构的多糖。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

Marx had to stand Hegel on his head, the mathematician Dirk Struik had to challenge his Platonist colleagues, and contemporary sociologists of knowledge still struggle to overcome Platonist myths and purist ideologies in science and mathematics.

马克思不得不站在黑格尔的思想之上，数学家史督克也不得不挑战他的柏拉图主义的同事，而当代的知识社会学家也必须努力克服科学和数学中的柏拉图神话以及纯化论的意识形态）。

The antineoplastic effective parts of Plumbago zeylanica L.were chloroform and petroleum ether extracts.

白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好，值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）有较好的抑制生长作用，ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml，对肝癌细胞hepg2抑制作用差， ic50为0.937 9 mg/ml；白花丹石油醚提取部位对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml；乙酸乙酯提取物对肺癌细胞（lu-04）有一定的抑制作用，ic50为0.734 3 mg/ml；水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好，值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

Man's first pure chemical means to create a life on: Poliomyelitis polio menu may be one of the most useful books.

人类首次用纯化学手段制造出了一种生命体：脊髓灰质炎（小儿麻痹菜谱也许是最实用的读物之一。

Man first pure chemical means to create a life on: Poliomyelitis polio menu may be one of the most useful books.

人类首次用纯化学手段制造出了一种生命体：脊髓灰质炎（小儿麻痹菜谱也许是最实用的读物之一。

Through the method, complete active pollen tube mitochondria is obtained, and the mitochondria separating and purifying efficiency is improved.

采用本方法获得完整的具有活力的花粉管的线粒体，提高了线粒体分离纯化效率。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。