纯化

The water-soluble holothurian glycosides were purified by macroporous resin and silica gel column chromatograghy, and the antifungal activities of different glycoside fractions were investigated.

先后利用大孔树脂柱层析和硅胶柱层析对水溶性海参皂苷进行了纯化，并对各种纯化组分的抗真菌活性进行了测定。

To exploit the highly effective natural antifungal medicaments from sea cucumbers, water-soluble holothurian glycosides from Apostichopus japonicus were isolated and purified, and their antifungal activities were examined.

从冻干仿刺参加工废弃液中分离纯化出水溶性海参皂苷，筛选并纯化出其强抗真菌活性组分，为利用水溶性海参皂苷研制高效抗真菌药物创造条件。

The recombinant baculovrius expressing the human antibody genes was isolated by co-transfection recombinant plasmid DNA with liner baculovirus DNA and purified by plaque assay, the intact recombinant human antibodies IgG-IV-2 and IgG-IV-6 was expressed in insert cells sf-9 after recombinant baculovrius infection and purified by protein G affinity chromatography.

通过空斑纯化，获得表达人抗体基因的重组杆状病毒，感染昆虫细胞进行抗体的全基因表达，表达产物经G-蛋白亲和层析纯化。

This paper develops the method of extraction and purification of thrombin from the porcine plasma by using Ion Exchange.

对离子交换法提取纯化凝血酶的方法进行了改进：首先在-20℃对猪血浆进行冷冻处理，然后再采用离子交换法提取纯化凝血酶。

First of all, this study will be multi-walled carbon nanotubes by chemical oxidation process purified so it can be modified to deal with than the purification of purity before the modified 5 wt%, more than 1.89 times the surface area to increase the surface potential decrease of about 10 ~ 20 mV and the surface functional base (-COOH and-OH) to increase 1.45 times, and then the control technology of electroless preparation parameters include: analysis of plating time, plating analysis of temperature, metal ions in solution than the (Fe2+/ Ni2+), bath pH and the added value of dispersion Agent and other research towards Fe-Ni particles to increase the iron content and Fe-Ni particles spread in the MWCNT upper fixed targets,the results found that when the parameters for the preparation of 50 ℃, pH10, do not add dispersant, metal ions than the solution (Fe2 +/ Ni2 +) 7, the highest iron content can be Fe = 40.55 at%(flat iron content 111.17 mg / g) of nanocomposites Fe-Ni/CNT, then for a series of the nature of its analysis, the final evaluation Fe-Ni/CNT nanocomposites were processed on the effectiveness of the application of mixed pollutants, the results showed that in 120 minutes at the same time when Adsorption of heavy metal ions lead nitrate 10 ppm, selenium heavy metal ion degradation of 1 ppm and orange azo dye AO7 50 ppm standard of effluents, and after a total Fe-Ni nano-particles to SEM / EDS analysis of more than 50% still remaining.

本研究首先将多壁奈米碳管以化学氧化法做纯化改质处理能使其纯度较纯化改质前提高5 wt％、比表面积提高1.89倍、表面电位下降约10~20 mV以及表面官能基提高1.45倍，再控制无电镀技术之制备参数包括：析镀时间、析镀温度，镀液中金属离子比(Fe2+/Ni2+)、镀液pH值及是否添加分散剂等，研究朝提高Fe-Ni粒子的含铁量与Fe-Ni粒子散布固定在MWCNT上等目标进行，研究结果发现当制备参数为50 ℃、pH10、不添加分散剂、镀液金属离子比(Fe2+/Ni2+)7时，可得到最高铁含量Fe = 40.55 at％(单位铁含量111.17 mg/g)之奈米复合材料Fe-Ni/CNT，接著对其作一系列性质分析，最后评估奈米复合材料Fe-Ni/CNT对共处理混合污染物之应用效益，结果显示其在120分钟时能够同时吸附重金属硝酸铅离子10 ppm、重金属硒酸根离子1 ppm及降解偶氮橘色染料AO7 50 ppm达放流水标准，且共处理后奈米Fe-Ni粒子以SEM/EDS分析仍剩余50 %以上。

Results Pseudomonas aeruginosa grew luxuriantly in carbon-filtrating water, soft water and purified water.

结果 纯化水制备工艺流程中的碳滤水、软化水及纯化水出口长满铜绿假单胞菌。

Xiangfan in Hubei-Portugal Biotechnology Engineering Company Limited is a production, processing, and sales of high-grade Portuguese Mannan, purification konjac flour, purified wild Caideng konjac gum and other agricultural products as one of the Export-oriented private enterprises.

湖北 襄樊惠葡生物科技工程有限公司是一家生产、加工、销售高纯度葡甘露聚糖、纯化魔芋精粉、纯化魔芋胶及山野菜等其它农副产品为一体的外向型民营企业。

This protein reduced antibody titer in mice immunized with BSA. Following the deletion of sCD152, however, there was no obvious inhibitory effect on the primary immune response triggered by the nonrelevant antigen OVA, indicating that sCD152 had an antigen-specific immunosuppressive effect. We designed a new vector, pPIC-HE-sCD152, to get entirely native sCD152 without His-tag.

为了获得既带有纯化配体表位又带有可剪切序列的可溶性sCD152蛋白，我们设计构建了含有His-tag和肠激酶识别切割序列DDDDK的sCD152表达载体pPIC-HE-sCD152，结果这一表达载体在毕赤氏酵母中同样高效分泌表达出目的蛋白，大量纯化后用肠激酶酶切去除His-tag，用以进一步功能实验，初步结果表明具有体外细胞免疫抑制功能。

In the experiment of department and purification,it is educated in 9K medium and Leathen medium as energy of thiobacillus ferrooxidans , it is adopted diluting and smearing slab to depart and purity, and introduced pabulum medium to prove the efficient of purification.

在分离纯化实验中，主要采用9K培养基和Leathen培养基作为T.f菌的生长能源，采用稀释涂布平板法来分离纯化该菌，并对分离后的菌液用牛肉膏蛋白胨培养基平板检验其分离效果。

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性，进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠，3次免疫小鼠后，血清(1：1000)中能检测到较强的MUC1抗体阳性；以最大OD450值之纯化血清做为一抗行蛋白印迹分析，结果显示：纯化血清可识别MUC1蛋白；小鼠脾脏淋巴细胞增殖率检测结果表明：噬菌体展示肽表位可在无佐剂存在情况下，大大促进小鼠的脾淋巴细胞的增殖，证明噬菌体是一较好的生物载体。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失