纯化

Two of these protein bands, with a molecular weight of 12 000 Mr and 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Caryota ochlandra. For the pollen extract of Carvota mitis, 30 protein bands were detected in SDS-PAGE, and three of these protein bands, with a molecular weight of 12000, 14000, and 26000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Carvota mitis. The 14000 Mr protein was the major allergen. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000、14000 Mr) from Caryota ochlandra were eluted in the second peak. The major pollen allergen of Carvota mitis was eluted in the forth and fifth peak.

结果 长穗鱼尾葵花粉有30余条蛋白带，其中主要条带有18条，12000和14000 Mr为长穗鱼尾葵花粉特异性变应原；短穗鱼尾葵花粉有30余条蛋白带，其中主要条带有9条，26000、1200和14000 Mr为短穗鱼尾葵花粉特异性变应原，其中14000 Mr为主要变应原；通过离子交换层析方法纯化出长穗鱼尾葵花粉相对分子质量为12000和14000的变应原主要分布在Ⅱ峰中，短穗鱼尾葵花粉相对分子质量为14000阳的主要变应原分布在Ⅴ峰和Ⅵ峰中。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen.

结果与结论：蜗轴组织细胞纯化后，胞体呈椭圆形或三角形，有细长的突起，Nuen染色胞核呈棕黄色阳性反应，β3-Tubulin染色细胞胞浆与轴突均呈棕黄色阳性反应。

RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen. Cytoplasm and axon were positive for β3-Tubulin.

结果与结论：蜗轴组织细胞纯化后，胞体呈椭圆形或三角形，有细长的突起，Nuen染色胞核呈棕黄色阳性反应，β3-Tubulin染色细胞胞浆与轴突均呈棕黄色阳性反应。

The study in this paper can not only provide a new method and an experimental foundation to further investigate molecular imprinted technology on separation and purification of MT, but also shorten production technology and promote btter application of MT.

本文的研究为进一步利用分子印迹技术进行金属硫蛋白的分离纯化、生产工艺的简化提供了一个新的方法，并为其更好的应用提供了实验基础。

According to the electrophilic substitution of α-Allocryptopin by ICl, it can be suggested that there are competitions of n-donors (such as N and O atom occurred in alkaloid or solvent molecules) with the conjugated π system in alkaloid molecule for iodine monochloride when charge-transfer complexes are formed, which may block the formation of pre-reactive π-complex intermediate, and thus suppress the electrophilic substitution.

这一方法标记过程简便，标记产物富集于有机相，易于进一步纯化，其放射化学纯度接近95%，标记率超过70%；ICl与α-别隐品碱的亲电碘化反应结果表明，生物碱底物或溶剂分子中的N、O等n电子给体可能与亲电试剂形成电荷转移络合物，竞争芳香亲电取代反应π络合物反应前体的形成，从而抑制碘化反应的发生。

Results GDNF plays extensive roles in the development and disease of motoneuron.

胶质细胞源性神经营养因子(glialcellline-derivedneurotropicfactor，GDNF)最初是从大鼠胶质细胞系B49的条件培养基中分离纯化获得的一个新的神经营养因子，GDNF为分泌性碱性蛋白质，在中枢神经系统和周围器官中有广泛分布，其表达受不同的转录因子等调控。

Mold of 34 species were isolated from moldy books.Among them,3 species which were demonstrated to be the key mould causing book mold were identified morphologically and molecularly as Chaetomium globosum,Aspergillus terreus and Aspergillus fumigatus.

从霉变的书籍中分离纯化了34种霉菌，对其中的3种主要霉菌进行了形态学和分子生物学鉴定，分别鉴定为球毛壳菌种、土曲霉种、烟曲霉种。

It shows us that the sufu can also treated as functional food. The biochemical process keeps unknown during sufu making. My study focuses on preparation, purification and hydrolysis properties of protease of Mucor piriformis Fischer which is a common used strain for process sufu. The main results are followed.

因此本论文选用用于腐乳生产的菌种梨形毛霉作为蛋白酶的生产菌种，从蛋白酶酶制剂的制备方法、性质的测定以及蛋白酶的分离纯化到该酶对大豆分离蛋白的降解作用逐一进行了研究，获得了以下主要结果。

Effects on sample to water, extracting temperature and extracting time for the polysaccharide yield, mucosity and surface tension were observed in normal extraction.

超声提取能提高PSK的得率，缩短提取时间，降低提取液的粘度和表面张力，有利于降低多糖浓度极化对分离纯化过程的阻力，提高处理量。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。