纯化

Seven prominent bacteria genera were identified through isolation and purification, among which Staphylococcus, Bacillus and Micrococcus genera were of the dominant bacteria in the fish pond.

通过对优势菌分离纯化，共鉴定得到7个属的优势菌属，其中葡萄球菌属、芽孢杆菌属和微球菌属为池塘中最主要的优势菌。

The expressed product was purified by affinity chromatography and identified by Western blot, then determined for enzyme activity by Micrococcus lysodeikticus turbidimetric method and for biological activity by heat shock method.

表达产物经亲和层析纯化后，进行Western blot鉴定，并用溶壁微球菌比浊法检测酶活性，热激法检测其生物学活性。

The CNTs were characterized with the transmission electron microscope and infrared spectroscopy. In addition, the dispersion of the CNTs in water was investigated with the optical microcopy and the viscosity meter. The FTIR spectrum experiments showed that there were many hydroxyl groups and carboxyl groups, on the surface of the acid-treated CNTs.

采用硫酸和硝酸混合酸对催化裂解法制备的多壁碳纳米管进行纯化，然后运用十六烷基三甲基溴化胺对CNTs进行修饰，并利用透射电子显微镜、红外光谱和拉曼光谱表征了CNTs的表面结构；另外，通过数码照相机和乌氏粘度计研究了修饰的CNTs在水中的分散性能。

7Kd. The minium activity of the catalytic antibody was detected to be 1～2μg/ml (10〓M) by the ELISA technique.

纯化后的酶性抗体经ELISA检测，其最低检出活性为1-2μg/ml。

Said method includes the following steps: firstly, adopting dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium EDTA, sodium carbonate and minor element solution A5 to prepare culture liquor of nostoc according to a certain ratio; then controlling temp. light intensity and deration condition of the culture liquor to make culture of nostoc; third ste, harvesting nostoc; fourth ste, extracting nostoc polysaccharide; and fifth step, purifying, harvesing and drying nostoc polysaccharide.

首先是配制葛仙米培养液：采用磷酸氢二钾、硫酸镁、氯化钙、柠檬酸、柠檬酸铁铵、乙二胺四乙酸二钠盐、碳酸钠，微量元素溶液A5按比例配制；其次是葛仙米的培养：控制温度、光强、光质、培养液通气条件；第三是葛仙米的收获；第四是葛仙米多糖的提取；第五是葛仙米多糖的纯化、收获与干燥。

A decade passed between the discovery of penicllin byAlexander Fleming and the development of a purification tech-nique by two British researchers that took the "miracle 0 drug"offthe shelf and placed it into medical practice,in time for use duringWorld War Ⅱ.

亚历山大·弗莱明发现青霉素之后又过了十年，才有两位英国医学研究人员研究出一种纯化的技术，将这种"神奇的药"从架子上拿下来用于临床医疗，恰好赶上在第二次世界大战中使用。

Objective To characterize and purify the pollen allergens isolated from Carvota mitis. and Caryota ochlandra.

目的 对长穗鱼尾葵和短穗鱼尾葵花粉变应原蛋白进行分析、鉴定与纯化。

Pollen allergens from Carvota mitts and Caryota ochlandra were purified and characterized. The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens from Carvota mitis and Caryota ochlandra.

对长穗和短穗鱼尾葵花粉变应原进行了初步的分离、鉴定和纯化，为临床鱼尾葵花粉变态反应疾病的诊断和治疗奠定了基础。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot.

重组短穗鱼尾葵花粉profilin在大肠杆菌中高效的表达，进一步经Ni2＋亲和层析柱纯化后经Western-blot检测具有良好的免疫学活性。

Methods The Streptococcus mitis isolated from patient's throat was shaking cultivated in Bact API 20 culture medium, the supernant fluid of which was precipitated with 20%、40%、60%、80%(NH4)2SO4, respectively. The fast protein liquid chromatography was used for final purification. The finally purified proteins were used to challenge rabbits suboutaneously and the responses of fever, diarrhea and reddening of the ears as well as mucous membranes were monitored.

将分离的缓症链球菌在Bact API 20培养基增菌培养后，所得上清液分别用20%、40%、60%、80%硫酸铵分段盐析，进一步快速蛋白液相色谱系统最终纯化，分别皮下注射新西兰兔观察有否体温升高、耳部及粘膜充血、腹泻等致热性表现。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。