纯化

Lymphotoxin Alpha ; High-Cell-Density Fermentation ; Annealing ; Purification

淋巴毒素α；高密度发酵；复性；纯化

The number of His-EGFP-CRP detection peaks increased after the protein was incubated with lysate of THP-1 cells.

为解决这一技术难题，构建了带his纯化标签和EGFP荧光标记的人CRP原核表达质粒［4］。

Objective To establish a feasible method for purifying tachyplesin from the wastes of tachypleus amebocyte lysate.

目的 建立从鲎试剂生产废料中纯化鲎素的方法。

Acquire newborn rat astroglial monolayer and dissect embryonic 18 d rat hippocampus and obtain single cell suspension through trypsin digestion. plate the suspension to poly l lysin coated coverglass in（2-5）×103 ?

首先以新生大鼠为实验材料培养纯化单层星形胶质细胞，随后以孕18 d胎鼠为实验材料，分离海马皮层，经胰酶消化制备单细胞悬液。

Acquire newborn rat astroglial monolayer and dissect embryonic 18 d rat hippocampus and obtain single cell suspension through trypsin digestion. Plate the suspension to poly L lysin coated coverglass in（2-5）×103 cm-2 density.

首先以新生大鼠为实验材料培养纯化单层星形胶质细胞，随后以孕18 d胎鼠为实验材料，分离海马皮层，经胰酶消化制备单细胞悬液。

Objective To optimize the procedure for purification and lysis of influenza virus vaccine.

目的 优化大规模生产的流感病毒裂解疫苗的纯化及裂解工艺。

A practical and feasible procedure for purification and lysis of influenza vaccine was developed.

已建立了切实可行的流感病毒裂解疫苗规模生产的纯化及裂解工艺。

Result and Conclusion: Macr oporous resins D301 can be used in the process of adsorbing and purifying active compounds of danshensu and protocatechualdehyde, and increase the amount of act ive compounds in water extracts.

结果 ：D301树脂能够有效的吸附丹参素和原儿茶醛。结论：采用D301树脂可以作为改进分离纯化工艺，去除杂质，提高粗提物中有效成分含量。

Besides, the polysaccharides were purified by thin-layer chromatography and its ingredients were mannose and glucose by composition analysis.

采用薄层层析法对胞壁多糖进行纯化，定性测得多糖的组分为甘露糖和葡萄糖。

The new antigen P3 peptide which harvested from the 430A peptide synthesizer were verified by the mass spectrograph and HPLC, that the ratio of mass/charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95%. The Western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22KD and 55KD proteins, which were existed in the testis tissue protein of mouse, rat and human.

一、经抗原分子设计，在430A自动肽合成仪上合成的新抗原P3，经质谱仪分析证明其荷质比与理论一致；纯化后，其纯度大于95％；将新抗原与KLH偶连后免疫小鼠，经Western blot证实其诱导的特异性抗体可识别小鼠、大鼠、人睾丸组织中分子量约为22KD和55KD左右的蛋白质。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。