纯化

A set of isolation and purification methods will be established, so it can be used for the separation of the trace amount compound.

同时建立一套分离纯化方法，使其能普遍应用于难于分离的微量化合物的分离纯化。

ObjectiveTo study the technological parameters of purification of total glycoside from Cornus officinalis with macroporous resin.

目的应用大孔吸附树脂对山茱萸中环烯醚萜苷类成分进行分离纯化，考察大孔树脂纯化山茱萸总苷的工艺参数。

In this thesis, using the PSP-producing dinoflagellate A/exandrium tarnarense, the technology on seperation and purification of PSP toxins have been studied and developed.

本文通过大量培养塔玛亚历山大藻来提取PSP毒素，完善了毒素的分离纯化过程，并对纯化毒素的活性进行了研究和探讨。

It was also used for the rapid separation and purification of lysozyme from egg white in only one step.

将其应用于鸡蛋清中溶菌酶的快速分离纯化，纯化后的溶菌酶纯度高于96%，比活高达71184 U/mg。

Satisfied the need of the research in preparation of fluorine by electrolyzation and the second-degree purifier further secured the whole operating system of the experiment besides purifying the fluorine gas.

实验研究表明：所研制的电解槽、化学冷却阱、吸收塔，防爆缓冲罐，尾气环保装置等设备设施可以满足本研究电解制氟的需要，且二级纯化装置不但具有纯化氟气的功效，而且还可对整个实验操作系统起到进一步的安全保障作用。

Results : The purity of the antibody was estimated to be greater than 90% after euglobulin precipitation combined with gel filtration, but less than 60% after ammonium sulfate precipitation combined with gel filtration.

腹水含有大量的杂蛋白，采用一步纯化法，不可能得到高纯度的IgM抗体，而多步骤纯化因耗时长，抗体活性损失大。

METHODS: HPMSCs were isolated from placenta of fetation human by density gradient centrifuge. Following bone marrow heparin anticoagulation, human BMSCs were isolated, purified and passaged by density gradient centrifuge.

采用密度梯度离心法从胎盘中分离、纯化和传代培养人胎盘源性间充质干细胞；骨髓肝素抗凝后，采用密度梯度离心法分离、纯化和传代培养人骨髓基质干细胞。

Bone marrow was obtained from healthy adult donors. METHODS: HPMSCs were isolated from placenta of fetation human by density gradient centrifuge. Following bone marrow heparin anticoagulation, human BMSCs were isolated, purified and passaged by density gradient centrifuge.

采用密度梯度离心法从胎盘中分离、纯化和传代培养人胎盘源性间充质干细胞；骨髓肝素抗凝后，采用密度梯度离心法分离、纯化和传代培养人骨髓基质干细胞。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Aim To optimize purification conditions of recombinant hirudin 3 in the fermentation broth and characterize the product.

目的优化分离纯化大肠杆菌发酵液中重组水蛭素Ⅲ的工艺并对所纯化产物进行分子鉴定。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失