纯化

The result showed that NS1 protein was highly expressed in a way of inclusion body.

用HisTrap HP Kit进行复性和纯化，获得了纯度较高、生物活性较好的NS1蛋白。

Objective To establish an effective method for purificating recombinant HspA-UreB fusion protein of Helicobacter pylori from inclusion body.

目的：建立一种有效的纯化幽门螺杆菌热休克蛋白A亚单位-尿素酶B亚单位融合蛋白包涵体的方法。

The way of preparing mouse Nanog fusion protein from the inclusion body was established, which was prepared for activation detection and mechanism of gene expression and regulation.

建立了一种可行的从包涵体中纯化重组小鼠Nanog蛋白的方法，为Nanog蛋白的活性检测及其基因表达调控机理的功能研究奠定基础。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in turbot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding trash fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病，常造成鱼类大规模的死亡。2006年6月山东某大菱鲆Scophthalmus maximus养殖场发生大规模腹水病，通过对病鱼细菌的分离、纯化和培养，对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查，将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较，并对其进行人工感染试验，结果证明这2株菌对健康大菱鲆都具有很强的致病性，而且感染症状完全一致。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in trubot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China. Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding raw fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病，常造成鱼类大规模的死亡。2006年6月山东某大菱鲆 Scophthalmus maximus 养殖场发生大规模腹水病，通过对病鱼细菌分离、纯化和培养，对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查，将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较，并对其进行人工感染试验，结果证明这2株菌对健康大菱鲆都具有很强的致病性，而且感染症状完全一致。

The P1 recombinant protein extracted and purified with B-PER GST Fusion Protein purification Kit was used as an antigen, and thallus component of full Mp was used as an antigen control to establish indirect ELISA experimental method to test and determine IgG antibody of serum samples from 40 normal blood donors and 51 clinical serum of partly certain and partly uncertain Mp infector.

以GST融合蛋白层析柱提取、纯化Mp P1重组蛋白做抗原，以全肺炎支原体菌体成分做抗原对照，建立间接ELISA实验方法，检测40份正常献血者血清标本和51份疑似MP感染临床血清标本的IgG抗体。

PAO polyclonal antibody was prepared by using recombinant human PAO protein which was purified by polyacrylamide gel electrophoresis as antigen to intradermally inoculate and immunize rabbit.

用制备性聚丙烯酰胺凝胶电泳分离纯化的重组人PAO蛋白，皮内接种日本大耳白兔以制备多克隆抗体。

To investigate its function, the Yeast invertase and the expressed protein were co-cultured

的全长编码序列，并通过大肠杆菌pMAL p2X 原核表达系统得到初步纯化的

Objective To explore a simple and rapid method for the isolation and purification of minipig islet cells.

目的 探索一种简单快速分离纯化小型猪胰岛的方法。

The best fermentation condition was chosen, and the antibacterial substance was isolated and extracted.

选择了适合该菌株的发酵条件，并通过硫酸铵沉淀、Sephadex G-50柱层析、高效液相色谱和质谱分析，对抗菌物质进行了分离纯化，并研究了其部分性质。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。