纯化

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

During that time, she often asked me the question related to PMA preparation by Microbiological Method and purification. I discovered that her ideas are so innovative, notwithstanding immatureness.

在那段时间中她经常问我微生物法制备PMA及其分离纯化这个目前国内研究的并不多的问题，虽然她的观点还很不成熟，但是我发现有些看法很有创新性。

The purified proteins were used to immunize Balb/c mice and to generate hybridomas.

得到的包涵体蛋白经纯化后免疫Balb/c小鼠并进行细胞融合。

The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody.

纯化的融合蛋白免疫新西兰大白兔制备多克隆抗体。

The expressed fusion protein and GST-VP3 were purified, and used as an immunogen to raise GST-VP3-specific polyclonal antibodies in male rabbits.

纯化表达的GST-VP2e融合蛋白，利用其作为免疫抗原，制备了兔抗GST-VP2e融合蛋白多克隆抗体，其效价达到1：512，000。

Aim To study the immunogenicity and safety of purified Vero cell rabies vaccine for human use.

目的研究人用狂犬病纯化疫苗的安全性和保护性。

The objective of the study was to investigate the industrial technology of preparing immunoglobulin with high purity and activity.

本研究目的在于研究适合工业化生产的纯化牛初乳中免疫球蛋白方法，得到高纯度高活性的免疫球蛋白产品。

However,there are a lot of problems unsettled by now in this field,such as the low efficiency of splicing in heterologous hosts,the low yield of intein mediated purification system and the impracticability of artificial control on the process of splicing.

但是，在目前的研究中，仍然存在着大量未解决的问题，如异源生物体内剪接效率低、蛋白质内含子纯化系统效率低以及不能实现蛋白质剪接人为控制等。

We observed in vitro and in vivo effects of the purified CTLA4Ig on T cell and its mechanism.

以此纯化的蛋白作用于体内、外，研究其对T淋巴细胞功能的影响及作用机制。

The complete labeling kits include unique purification columns that ensure the complete removal of unreacted dye while yielding very high sample recovery, independent of fragment length.

完整的标记试剂盒包括通用纯化柱能够确保完全去除未反应的染料，并进行高质量的样本修复，而无论片段长度。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。