纯化

Effects of three factors on the antimicrobial activity of antimicrobial substances in the hemolymph from Ruditapes philippinarum were studied by the method of orthogonal design.

为了建立蛤仔血淋巴中抑菌活性物质的快速高效分离、纯化方法，利用正交设计法对影响菲律宾蛤仔血淋巴中抑菌物质抑菌活性的3个因素进行了探讨。

Experimental animals were randomly divided into control group and hepatocirrhosis groups.

首先行大鼠mscs的分离、纯化及扩增。

The research of the characteristics of biology and the osteogenic and adopocitic induction are comparatively mature, showing many biological characteristics such as adherence, plasticity, heterology, self-renewal, rapid clone formation and transplantation. Adherence combined with density gradient centrifugation method is widely used in the isolation and purification of BMSCs.

骨髓间充质干细胞现阶段研究方向主要包括生物学特性、诱导分化和调控机制等内容，对其生物学特性和成骨成脂诱导方面研究比较成熟，在培养分化过程中展示出贴壁性、可塑性、异质性、自我更新能力、快速形成克隆能力、可移植性等生物学特征，贴壁法联合密度梯度离心法在骨髓间充质干细胞的分离、纯化过程中被广泛采用。

Results:92% purity of hirudin was obtained after the separation purification.

结果：经过分离纯化可以得到纯度为92%的水蛭素。

Finally the high expression and low degradation of hirudin were achieved.

在分离纯化并鉴定出水蛭素的四种活性异构体后，通过恒化培养的研究探索影响水蛭素降解的因素，最终在分批发酵中实现水蛭素的低降解高表达。

To set up the method to isolate and purify rat bone marrow mesenchymal stem cells and to investigate the inducing effect of injured liver tissue homogenate.

目的：建立分离、纯化大鼠骨髓间充质干细胞的方法，探讨损伤肝组织匀浆对MSCs的诱导分化作用。

The molecular weight of GST-GFP was 53kDa in homonymous to its theoretical size. GFP was purified by using affinity binding and thrombin treatment.

GST-GFP分子量大约为53kDa，与其理论值大小一致，用亲和层析以及凝血酶处理纯化GFP。

Balb/c mice were immunized with the purified PRRSV - BJ isolate. mouse spleen cell were fused with myelomas NSo .culture supernatants of hybridism's were screed by indirect ELISA. Positive colonies were cloned three times by limited dilution. One cell line named 6PR, which could secret McAb stably was obtained.

用纯化的猪繁殖与呼吸系统综合征病毒免疫Balb/c小鼠，应用淋巴细胞杂交瘤技术，取脾细胞与NS0骨髓瘤细胞融合，经间接ELISA筛选，三次有限稀释法克隆，得到一株能稳定分泌抗猪繁殖与呼吸系统综合征病毒单克隆抗体的杂交瘤细胞株：6PR。

Isolation, purification and characteristics of D-hydantoinase were carried out. The experiment results showed that the optimal pH and temperature of pure D-hydantoinase were pH 8. 5 and 35℃, respectively. D-hydantoinase activity could be increased significantly by Mn〓 and Fe〓. Substrate specificity of D-hydantoinase on various hydantoin derivatives showed the optimal substrate of D-hydantoinase was 5-phenylhydantoin, but hydantoin, 5-methylthioehylhydantoin, 5-hydroxyphenylhydantoin, 5-methylhydantoin, 5-benzylhydantoin could also be hydrolyzed by D-hydantoinase.

本文还进行了海因酶的分离纯化及性质研究，发现酶的最适反应温度为35℃，最适pH为8.5，金属离子Mn〓和Fe〓能显著提高D-海因酶活力。D-海因酶的特异性实验表明，D-海因酶的最适底物为D，L-苯海因，其次为海因，D，L-对羟基苯海因和D，L-甲硫乙基海因，对D，L-甲基海因和D，L-苯甲基海因也有一定的作用。

Graded hydrolysates were desalted by cation ion- exchange resins and anion ion- exchange resins. By comparing the desalting rate and peptide recovery rate, the optimum hydrolyte flow rate was 10 CV/h.

采用阴阳离子交换树脂脱盐法对超滤分级肽进行纯化，以肽回收率和脱盐率为考察指标，确定10 CV／h为最佳流速。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。