纯化

Macroporous adhesive resin column and Sephadex LH20 column were employed to separate and fractionate the crude extract and the structures of the main compounds were analyzed by HPLC-ESI-MS.

通过大孔树脂结合SephadexLH—20对芒果多酚粗提物进行分离纯化和分级制备，采用HPLC-ESI-MS对主要组分的结构做了初步分析。

However, parkour purists feel that the direction in which Foucan has taken the activity is entirely different (in both execution and philosophy) from what he and Belle had originally developed—and that what is now known as free-running should not be confused with "true" parkour.

但是，跑酷纯化论者觉得福冈为跑酷指出的方向与他和贝尔最初的设想大相径庭，因此目前所谓的自由奔跑不应与"真正"跑酷相混淆。

Purified proteins were mixed with Freund's adjuvant and then were used to immunize rabbits.

将纯化后的GST-R185、GST-R150和GST蛋白免疫新西兰大白兔制备抗血清。

This technique was modified as follows: the small rubber particles were purified and diluted to give a protein concentration of an absorbance at 600 nm of about 2.0～2.5; 0.5% basic fuchsin was prepared by being dissolved in 50 mmol/L Tris-HCl(pH7.4); the reaction mixtures were incubated for 30 min at 25 ℃, and centrifuged for 5 min at a speed of 5 000 r/min; and B-serum can serve as the protein samples for the positive control of rubber particle aggregation.

几个关键参数为：（1）使用纯化的小橡胶粒子，适宜浓度为在600 nm处的OD值为2.0～2.5；（2）用50 mmol/L Tris-HCl（pH7.4）配制0.5％碱性品红；（3）25 ℃条件下孵育30 min；（4）5 000 r/min离心5 min；（5）B乳清可作为阳性对照的蛋白质样品。

This experiment, the extract from fresh seaweed polysaccharide reference thermal flooding of alcohol, iodine waste material after the extraction of crude to fucose sulfate polysaccharide extraction, classification after the ethanol precipitation method used for crude and industrial pre-kelp dealing with separation of water purification, and anthrone-sulfuric acid method of its total sugar content.

本实验，采用从鲜海带中提取多糖的热水浸提法对醇、碘提取后的废弃料进行粗品岩藻多糖硫酸酯的提取，后采用乙醇分级沉淀法对粗品和工业海带预处理水进行纯化分离，并用硫酸蒽酮法测定其总含糖量。

To determinant the functional motif of S protein involved in the interaction with ACE2, seven truncated S proteins deleted from N or C terminal were obtained by E.

为了确定S蛋白与ACE2结合的功能区域，我们在大肠杆菌中表达了7个从N端截短或从C端截短的S蛋白，并用Ni柱纯化。

We designed four chips for different functions, fundamental separation fixed quantity chip, parallel separation fixed quantity chip, series separation fixed quantity chip, and combinative separation fixed quantity chip.

由此理论公式吾人设计了四种具不同功能功能性定量流体碟片，包括流体分流定量碟片、平行定量碟片、依序定量碟片、二次样品纯化定量碟片，经实验后其定量功能符合吾人所需功能。

Fusarium oxysporum from wilt diseases in Guangxi hybrid bamboo were separated and purified, and the biological characteristics of the pathogen were studied.

分离纯化了广西杂交竹枯萎病原镰刀属尖孢镰刀菌，并研究了该病原菌的生物学特性。

This means of affinity chromatography with gallinaceous ovomucoid has been proven to be a kind of economical and applicable method to purify and isolate WGA.

鸡卵黏蛋白亲和色谱法是一种经济适用的纯化麦胚凝集素的好方法。

Methods Chromatographic column coupled with gallinaceous ovomucoid was used to isolate and purify WGA.

先制备鸡卵黏蛋白的亲和吸附剂，然后用其纯化麦胚凝集素。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失