纯化

The technology of molecular-distillation for purifying star anise essential oil was researched, and chemical constitutions of essential oil and distilled substance were analyzed by GC-MS.

研究分子蒸馏技术纯化八角精油的工艺条件，利用GC-MS对精油及分离纯化的馏分进行化学组成分析。

In this study, we isolate, purify and characterize endo-β-glucanase by Ascomycete Xylaria regalis. The cellulose-containing medium is used to induce the production of the cellulose-degrading enzymes. When these enzymes are produced at maximum level, we harvest and filtrate the culture medium. The filtrate is purified by anion exchanger column and hydrophobic interaction column (phenyl-650M). The purification fold of endo-β- glucanase increases 4.3 times, the specific activity approaches 3.7 unit/mg, and the recovery is 27.6%, respectively.

为了分离且纯化此株菌产生在培养基中的endo-β-glucanase，先将此菌在cellulose液态培养基中大量培养，在此酵素达到最高峰时，利用滤纸过滤将菌丝及菌液分开，收集粗酵素液，再经过阴离子交换树脂及疏水性层析管柱(Phenyl-650M)等步骤予以纯化，所得到最终酵素纯度为 4.3倍，比活性为3.7 unit/mg，回收率为27.6%。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

With the development of the biotechnology, a lot of advanced techniques for separating bioactive substance such a...

随着生物技术的发展，蛋白质的分离纯化技术在近几年得到了巨大的进步，许多新的纯化技术相继问世。

With the development of the biotechnology, a lot of advanced techniques for separating bioactive substance such as recombinant proteins have been established.

随着生物技术的发展，蛋白质的分离纯化技术在近几年得到了巨大的进步，许多新的纯化技术相继问世。

Firstly, XRD, TEM and FT-IR were used to characterize the structure of CNTs treated by dense HC1, dense HNO_3, HNO_3, H_2SO_4-HNO_3, K_2Cr_2O_7-H_2SO_4 and bromic acid. The results showed that purification treatment had no change on the structure of CNTs. But on the top of CNTs have function group such as -OH and -COOH after H_2SO_4-HNO_3 and K_2Cr_2O_7-H_2SO_4 were treated.

采用浓HCl、浓HNO_3、H_2SO_4-HNO_3、K_2Cr_2O_7-H_2SO_4、溴水共5组物质分别对CNTs进行纯化处理；借助XRD、TEM、FT-IR等分析手段对处理前后CNTs结构及形貌进行表征，发现以上5种物质纯化处理并不会对CNTs结构造成很大影响，其中H_2SO_4-HNO_3和K_2Cr_2O_7-H_2SO_4处理效果较好，除杂的同时CNTs端口带上-OH或-COOH活性官能团。

The methods of purification and reconstitution of Ca〓-Mg〓-ATPase were set up and Ca〓-Mg〓-ATPase from both spontaneously hypertensive rat and control rat were isolated, purified and reconstituted in the study.

建立了质膜钙泵的分离纯化方法和磷脂重建方法并用这些方法成功地分离纯化了正常大鼠和自发性高血压大鼠红细胞膜钙泵，而且将其重建于人工脂质体上。

However, CDH has little synergism with cellobiohydrolase or endoglucanase in cellulose degradation. But, if the substrate was pretreated with CDH in the presence of cellobiose, the rate of the hydrolysis of the substrate by cellobiohydrolase or endoglucanase increased.

CDH与纯化的外切纤维素酶或内切纤维素酶或内切纤维素酶之间没有协同作用关系，但在纤维二糖存在条件下底物事先用CDH处理后再用上述纯化的酶水解，作用明显增强。

As for purification, there was no significant difference between filter and centrifugation methods.

对比离心纯化与过滤纯化，并未发现两者之间的差异。

The expressed products were purified by Glutathion-Sepnarose chelation affinity chromatography and tested by western blot.

对表达的蛋白进行Western blot分析和谷胱甘肽琼脂糖亲和层析纯化，得到纯化的VP4融合蛋白。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失