纯化

The author first reported the social phenomenon and synchronizing development incryptosporidia,the dynamic process of the sporozoites or merozoites aparted from theirparent substances and developed into trophozoites,the culture of C.baileyi in chickenembryo,and the excysting process without both the trypsin and the sodium taurocholatein 37℃ water.The high levels of excystation(60%)oceured at 37℃ water showedthat temprature was very important in cryptosporidia excysting process.

在国内首次观察和描述了薄壁型隐孢子虫卵囊；首次报道隐孢子虫具有群体寄生现象和同步发育特征；首次描述了隐孢子虫的裂殖子或子孢子离开母体和发育为滋养体的动态变化过程；首次应用粪便中纯化的C.baileyi卵囊感染鸡胚成功，并用37℃水脱囊，其脱囊率高达60％，表明温度对隐孢子虫的脱囊具有重要作用，无需胰酶和牛胆酸钠的参与。

AB-8 have an upper adsorption capacity for polysaccharides in exocarp of Ginkgo biloba L and the elution is easy.

AB-8树脂吸附性较好，多糖洗脱容易，纯化后多糖含量达81.3％。

Coli strain stably and highly expressing recombinant Pseudomonas aeruginosa exotoxin A and for purification of expressed product.

目的 建立稳定、高效表达重组铜绿假单胞菌外毒素A的工程菌发酵及表达产物纯化工艺。

Objective In order to isolate and purify an exotoxin of Streptococcus mitis which can cause toxic shock syndrome and investigate its pathogenicity.

目的 分离与纯化致中毒性休克综合征缓症链球菌外毒素蛋白，并对其致病性进行初步研究。

Bacillus thuringiensis ;β-exotoxin ; Two-phase aqueous system ; Isolation and purification

苏云金杆菌；β-外毒素；双水相系统；分离纯化

The general quantities of organic acids in root exudate under sand culture conditionwas two times higher compared to those in solution culture.

机械阻力促进了有机酸特别是苹果酸的释放，砂培试验根分泌有机酸的总量是溶液培养试验的3倍，用阴阳离子树脂纯化根分泌物中的有机酸，有利于液相色谱的测定。

In this paper, the methods for the study of root exudates, including collection, isolation, purification and identification, are reviewed.

本文着重讨论根系分泌物收集、分离纯化及鉴定的常用方法，并列举特定根系分泌物的研究方法，旨在为根系分泌物的研究提供借鉴和参考。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼，红文鱼，红顶白高头，白珍珠，红狮头，红龙睛蝶尾，墨龙睛，红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列，PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列，在129个样本中共检测到了17个单倍型，9个品种共享1种单倍型，5个品种的金鱼(BM， MB， WR， RF， RC)检测到各自独有的单倍型。

In this report, we prepared antiserum to pV of Ad41 and studied the mechanism of Ad41 fastidiousness.

本研究拟表达纯化Ad41 pV抗原，免疫动物制备抗血清，为研究Ad41难养性机理打下基础。

Methods Mesenchymal stem cells were collected from the femora of SD rats by density gradient centrifugation and identified by flow cytometric analysis and alkaline phosphatase staining.

方法采用梯度离心法，分离纯化SD大鼠的MSCs，流式细胞仪检测和碱性磷酸酶染色鉴定细胞类型。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失