纯化
- 与 纯化 相关的网络例句 [注:此内容来源于网络,仅供参考]
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A PCR product was amplified using RT-PCR method from total RNA from the abdomens of R strain using the degerated primer. The PCR product was purificated and cloned. The plasmid is pBluescript Ⅱ KS. Recombined plasmid DNA was identified by endoenzyme. The results indicated there were different P450 genes in the PCR product.
运用这对简并引物首次从德国小蠊抗拟除虫菊酯的品系R中扩增出的特异性的DNA片段,经纯化、连接和转化,重组至pBS质粒,筛选阳性克隆,提取重组质粒的DNA,并经初步的酶切鉴定,发现存在着不同的CYP4基因。
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The molecule engram polymer is characterized by simple, fast, high-effective separation and purification, enriching residual alserin, and direct usage for selective separation and enrichment alserin.
本发明制得的分子印迹聚合物能够简单、快速、高效分离纯化、富集生物样品中的利血平残留,可直接用于对利血平的特异选择性分离、高效富集。
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The molecular weigt of Acetobacter Z127 ADH was 28.0 KDa judged by SDS-PAGE which was similar to the Entamoeba histolytica ADH(31.0 KDa) reported.4 Research on parts of ADH properties.
Acetobacter Z127中的ADH经细菌裂解液粗提、硫酸铵盐析精提、葡聚糖凝胶过滤层析三步处理纯化了5.49倍,酶活收率为48.09%。
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After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.
经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品,并对融合蛋白的肠激酶切割特性进行了初步研究。
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In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.
利用肠激酶加工融合蛋白以制备rhIL-11的方法简单可行,分离纯化效果好,能够满足工业生产的需要。
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Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.
经肠激酶切割和纯化,获得了与天然蛋白N-末端相同的hK5重组蛋白。
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Having been renaturalized, the fusing protein product is digested with enterokinase, purified by Ni-sepharose and HPLC.
复性、肠激酶酶切、Ni-Sepharose和HPLC纯化后,用质谱测定其分子量并进行活性测定。
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In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.
此外类凝血酶基因r8在pBAD/TOPO中也得到高效融合表达,表达产物经酶切、纯化和复性,测得精氨酸酯酶活性。
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The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.
纯化后的融合蛋白经肠激酶切割和回收后,所得目的蛋白(IL-29)纯度大于96%,该蛋白N-端序列与理论值一致,其抗病毒活性与IFN-α2b相当。
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Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.
采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA,将其克隆入pET32a原核表达载体中,并在大肠杆菌BL21(DE3)中进行表达,然后以镍亲和层析法对表达产物进行纯化。
- 推荐网络例句
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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.
每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。
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Maybe they'll disappear into a pothole.
也许他们将在壶穴里消失
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But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.
但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。