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纯化

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A PCR product was amplified using RT-PCR method from total RNA from the abdomens of R strain using the degerated primer. The PCR product was purificated and cloned. The plasmid is pBluescript Ⅱ KS. Recombined plasmid DNA was identified by endoenzyme. The results indicated there were different P450 genes in the PCR product.

运用这对简并引物首次从德国小蠊抗拟除虫菊酯的品系R中扩增出的特异性的DNA片段,经纯化、连接和转化,重组至pBS质粒,筛选阳性克隆,提取重组质粒的DNA,并经初步的酶切鉴定,发现存在着不同的CYP4基因。

The molecule engram polymer is characterized by simple, fast, high-effective separation and purification, enriching residual alserin, and direct usage for selective separation and enrichment alserin.

本发明制得的分子印迹聚合物能够简单、快速、高效分离纯化、富集生物样品中的利血平残留,可直接用于对利血平的特异选择性分离、高效富集。

The molecular weigt of Acetobacter Z127 ADH was 28.0 KDa judged by SDS-PAGE which was similar to the Entamoeba histolytica ADH(31.0 KDa) reported.4 Research on parts of ADH properties.

Acetobacter Z127中的ADH经细菌裂解液粗提、硫酸铵盐析精提、葡聚糖凝胶过滤层析三步处理纯化了5.49倍,酶活收率为48.09%。

After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.

经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品,并对融合蛋白的肠激酶切割特性进行了初步研究。

In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.

利用肠激酶加工融合蛋白以制备rhIL-11的方法简单可行,分离纯化效果好,能够满足工业生产的需要。

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化,获得了与天然蛋白N-末端相同的hK5重组蛋白。

Having been renaturalized, the fusing protein product is digested with enterokinase, purified by Ni-sepharose and HPLC.

复性、肠激酶酶切、Ni-Sepharose和HPLC纯化后,用质谱测定其分子量并进行活性测定。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD/TOPO中也得到高效融合表达,表达产物经酶切、纯化和复性,测得精氨酸酯酶活性。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后,所得目的蛋白(IL-29)纯度大于96%,该蛋白N-端序列与理论值一致,其抗病毒活性与IFN-α2b相当。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA,将其克隆入pET32a原核表达载体中,并在大肠杆菌BL21(DE3)中进行表达,然后以镍亲和层析法对表达产物进行纯化

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。