纯化

ObjectiveTo establish a feasible method of culture and purification of embryonic dorsal root ganglion neurons of rats.

目的：建立一种切实可行的胚胎大鼠背根神经节神经元培养及纯化方法。

And now the research of the application of PNA have been the hotspot of gene druggery treatment, anti-gene and anti-sense strategy.

现在肽核酸在基因药物治疗，反基因、反义策略，基因定位，核酸纯化等广泛领域越来越显示出其优越的特性。

We purified the target protein under natural conditions by MCAC through Ni-NTA resin. The purity of this fusion PH domain of IRS-1 was up to 98％. We paned out the oligopeptides that can bind to this target protein from a phage displayed random duodecimal peptide library. The identities of peptide sequences of positive clones were analyzed.

因此，我们利用融合表达载体pRSET在T7启动子的调控下表达出氨基端带六个连续组氨酸标签的IRS-1的PH结构域，利用Ni-NTA介质在非变性条件下对目的蛋白进行了金属螯合亲和层析纯化，获得了纯度达98％以上的PH结构域融和蛋白，以此为靶分子，从噬菌体展示随机12肽库中筛选出能够与其相结合的短肽，并分析了阳性克隆的序列一致性。

The molecular weights of the 11 major bands were 82, 79, 60, 51, 46, 38, 32, 30, 28, 24 and 18kD, respectively. The results of Western blotting with the positive serum of anaphylactic patients showed that the 1st-4th instar larvae all had the specific allergens whose molecular weights were 82 and 79kD, respectively; only the 5th instar larvae all had the specific allergen of 30kD. We purified the protein of 30kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.

选用家蚕过敏患者阳性血清进行免疫印迹，1~4龄家蚕均显示出82和79kD的特异性变应原；但只有5龄家蚕的30kD蛋白为特异性变应原，通过离子交换层析和经切胶纯化出30kD蛋白，再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。

Among them, McAb-14, McAb-18 and McAb-21 had strong neutralization characteristics, and there was strong correspondence between the neutralization efficacity and the haemagglutination inhibition value.

应用杂交瘤技术，以纯化的NDV-lasota为免疫原结合间接ELISA筛选到了24株抗NDV单抗，其中3株（McAb-14、MoAb-18和McAb-21）具有中和活性，并与其血凝抑制效价具有很大的相关性。

The separation and purification of Immunoglobulin of egg yolk were studied.

对鸡卵黄免疫球蛋白的分离纯化进行了研究。

Objective: To develop a method for preparation of coating antigen in measles IgG EIA test system.

目的：摸索纯化麻疹病毒的方法，并以其为包被抗原，制备麻疹IgG酶联免疫诊断试剂。

With the purified α-NT-linked affinity chromatography, nicotinic acetylcholine receptor is purified from the electric organ of ray .

以α-NT制作亲和胶柱，纯化了黑斑双鳍电鳐电器官中的烟碱型乙酰胆碱受体。

In the meantime, all the water used in the process has undergone electrodialysis, ion exchange, germicidal treatment, and is purified water.

同时，全部工艺用水经电渗析、离子交换、灭菌处理后得到的纯化水。

Three method, including Percoll density, centrifugal elutriation and plate method, for enrichment of PGP9.5 cells were contrasted.

为了提高纯化方法富集PGP9.5 细胞的效率，本试验对比了Percoll法、差速贴壁法和盘化法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失