纯化

Wu39-1,Wu43-1 and Wu50-2 were used to develop a handy extraction method of mtDNA in crucifer s on the basis of the sucrose-bedding method of mtDNA extraction and by means of four combinations of different centrifugal forces,differential centrifugation,and composition adjustment and dosage increase of buffer solution A.

以十字花科植物芸芥 Eruca sativa 、白菜型油菜 Brassica rapa 和甘蓝型油菜 Brassica napus ：武39- 1、武4 3- 1、武5 0 - 2为试材，在蔗糖衬垫法分离纯化m t DNA方法优点的基础上，通过4组不同离心力配比，采用差速离心法、改变缓冲液A的成分以及增加缓冲液的用量，建立了一种简便提取十字花科植物mt DNA方法。

The expression plasmid pGEX-5X-1/DFF45 was constructed and transformed into E.coli BL21, and the expression of GST-DFF45 protein was induced by IPTG. After purification, the fusion protein GST-DFF45 was used to immunize Oryctolagus cuniculus to obtain the antibody serum.

构建pGEX-5X-1/DFF45原核表达质粒，转化大肠杆菌BL21，用IPTG诱导融合蛋白表达，经纯化后免疫日本大耳白兔得到多克隆抗体并用CNBr-activated Sepharose？

Erythrocytes of sika deer blood ; cupper and zinc superoxide dismutase ; purification ; property

鹿血红细胞；铜锌超氧化物歧化酶；纯化；性质

Research of changes in the mechanical properties and curability of composite core materials modified with ultrafine diamond

介绍了爆轰法合成纳米金刚石在理论、制备及合成技术研究的现状，指出了影响纳米金刚石生产成本及制约其工业应用的关键在于爆轰产物的纯化和分散。

METHODS: Affinity column constituted by gelatin coupling with sepharose 4B activated with cyanogen bromide was used to purify fibronectin.

用明胶与溴化氰活化琼脂糖珠偶联的亲和层析柱对纤维蛋白结合素进行纯化。

So, it is very important to synthesize cyclic peptides by chemical method.

尽管从自然界中分离鉴定了很多具有生物活性的环肽，但含量甚微，分离纯化困难。

Methods: Two sets of primers were designed according to the cyclostyle of pcDNA3.1-HBV-YVDD, and the full-length of HBV genome with different enzyme digestin spot was amplified.

以pcDNA3.1-HBV-YVDD为模板设计两对引物，扩增带不同酶切位点的HBV全基因，扩增产物纯化后，经酶切、连接，得到pcDNA3.1-HBV-YVDD重组体。

Purpose To compare the effects of three different cation exchange resins for purifying Cytochrome C.

目的 比较3种不同树脂纯化细胞色素C的效果。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中，经39000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失