纯化

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因，鉴於intersex在果蝇中所扮演性别决定的重要功能，对於此基因在鲤鱼中所担任的功能而感到兴趣，为了进一步探讨cIX的功能，本论文完成cIX全长cDNA，设计并建构cIX的重组DNA，再利用纯化之重组蛋白诱导抗体。

The separation mechanisms of powder quartz and clinochlore as well as the adsorption mechanism of the anionic collector were investigated.

采用油酸作阴离子捕收剂，用反浮选的方法对粉石英的纯化进行研究，探讨粉石英和斜绿泥石的分离机理以及捕收剂的吸附机理。

The purification of powder quartz was studied through reverse flotation by using oleic acid as the anionic collector. The separation mechanisms of powder quartz and clinochlore as well as the adsorption mechanism of the anionic collector were investigated.

采用油酸作阴离子捕收剂，用反浮选的方法对粉石英的纯化进行研究，探讨粉石英和斜绿泥石的分离机理以及捕收剂的吸附机理。

Objective To purify coagulase from the filtrate of Staphylococcus aureus and analyze its property and function.

目的 对金黄色葡萄球菌滤液制剂中凝固酶进行纯化，并对其性质及作用进行分析。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Cochineal wax separation and purification Abstract: Dactylopius coccus Costa is an extremely valuable resource insects, is of great economic value.

胭脂虫蜡的分离及纯化摘要：胭脂虫是一种极其珍贵的资源昆虫，具有重大的经济利用价值。

Separation and purification of Human-like collagen I and its functional properties were researched in this paper.

本文对类人胶原蛋白 I的分离纯化及其功能特性进行了研究。

METHODS Retinal cells were cultured in commixture and retinal ganglion cells in pu-rification,to observe the state of ganglion cells and its axon.RESULTS Retinal ganglion cells that cultured in commix-ture grew very well.

进行猪视网膜细胞混合体外培养和神经节细胞的纯化培养，观察神经节细胞生长状态及轴突生长情况，研究视网膜不同类型神经细胞之间的相互作用。

METHODS Retinal cells were cultured in commixture and retinal ganglion cells in purification, to observe the state of ganglion cells and its axon. RESULTS Retinal ganglion cells that cultured in commixture grew very well.

方法进行猪视网膜细胞混合体外培养和神经节细胞的纯化培养，观察神经节细胞生长状态及轴突生长情况，研究视网膜不同类型神经细胞之间的相互作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失