纯化

A new type of separation and purification method for isolating puerarin was developed using chelate complex chromatography.

中文内容：摘要：建立了采用配位色谱柱从葛根素浸膏中分离纯化葛根素的方法。

HARD1 protein was expressed by inducing with IPTG(1 mmol/L) and purified up to 95% through Ni2+ chelation affinity chromatography.

以纯化的重组蛋白免疫小鼠，制备了抗hARD1蛋白的抗血清。

Western Blotting experiments shown that the CspA protein of Bacillus stearothermophilus can be expressed under the condition of not only cold shock but also the induction of Chloromycetin.

用纯化的蛋白质免疫兔子获得抗血清，Western Blotting表明CspA蛋白于嗜热脂肪芽孢杆菌中不仅冷激时表达，而且在受到氯霉素诱导时也会表达。

104S-1e.The strains of bacteria identified as Lactobacillus were isolated from yoghourt, pickle and intestine of domestic animal such as chook, duck and rabbit.

从畜禽鸡鸭和兔的肠道、酸奶及泡菜中分离纯化得到四株转化CLA的菌株，初步鉴定均为乳杆菌。

The synthesized peptide was purified by the chromatograph and identified by mass spectrograph.

用色谱仪和质谱仪对合成的多肽进行纯化、鉴定。

The purified N protein was used to coat 96-well plate, each step was optimized, such as coating concentration of recombinant nucleocapsid protein, scample dilutim, chromogen and stop solution as well as concentration.

在此基础上，以纯化的重组N蛋白作为包被抗原，对各种条件进行优化（如抗原的包被，作用时间及底物的选择），确定了判定标准，建立了检测PRRS抗体的间接ELISA方法。

Through extraction, separation, purification and structural identification, the functional compound in Chufa husk was found to be the same compound as that in waste slurry.

再次通过分离纯化、气质联用分析荸荠皮中的功能物质，发现其主要成分和实验中从产粉废浆中提取的功能因子相同。

Also the molecular structure and functional effects of this active component were studied in detail. 1 Extraction, separation, purification and structural identification of the functional component in Chufa After initial handling of waste slurry to solid powder, ethanol was used to lixiviate it followed by the extraction with petroleum ether,ethyl acetate,n-butyl alcohol one by one, the polarity of three organic reagents being changed from low to high.

荸荠功能因子的提取、分离、纯化及结构鉴定以马蹄粉厂产粉废浆为原料，经过实验室初处理成固态原料粉后，用乙醇浸提，再将浸提物以水悬浮，依次通过极性从小到大的石油醚、乙酸乙酯、正丁醇三种有机溶剂两相萃取分部，并以金黄葡萄球菌、枯草芽孢杆菌和致病大肠杆菌为指示菌，检测上述各部分提取物的功能效果。

The solubilized Cry8Ca2 protoxin of 130kDa was activated by chymotrypsin at the enzyme / protoxin ratio of 1∶20.

用原毒素与纯化的毒素对铜绿丽金龟幼虫进行生测，Cry8Ca2原毒素蛋白的LC50为0。

Manual microdissection in the isolation and purification of ciliated cells, can reduce the interference of impure cells, increase the cell activity, and is a satisfied technique to offer pure cells.

将手工显微分离技术应用于纤毛细胞分离和纯化降低了杂质细胞干扰，提高了细胞活性，满足了提供纯净目的细胞的实验要求。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失