纯化

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

A water soluble polysaccharide was isolated and purified from the dry leaves of bamboo that grows in Hubei Province.

采用纸色谱和气相色谱法研究了从湖北恩施地区生长的箬竹叶中分离纯化的水溶性多糖的单糖组成。

When the first purification alcohol extract first enrichment, then add water to dissolve acid, then the acid fluid, finally using organic solvent basification extraction, the organic solvent extraction layer to get concentration, total alkaloid.

纯化时首先把醇提取液先浓缩，然后加入酸水进行溶解，接着再把得到的酸水液碱化，最后用有机溶剂萃取，萃取得到的有机溶剂层再进行浓缩，就得到总生物碱。

The notion that a functioning state must rely upon pure ethnicity and a set of cultural and religious beliefs widely and deeply shared by those within its boundaries may be one of the most destructive ideals of the last millennium.

认为一个有效运行的国家必须依赖纯化的种族以及一整套被国民所普遍接受的宗教与文化信仰，这种观点或许是上一个千年中最具有破坏性的想法之一。

The method is simple and rapid with high recovery of lignans and can be used for the preparation in large scale.

用大孔树脂提取和纯化五味子总木脂素的方法在保留五味子中有效成分的同时，显著降低固形物得率，可用于工业推广。

We compared the effect of sevoflurane on ciliary beat frequency with that of halothane and isoflurane using purified andcultured rat tracheal epithelial cells.

我们用纯化培养的大鼠气管上皮细胞比较了七氟烷与氟烷和异氟烷对纤毛活动频率的影响。

Synthesis technology and purification method and cleaner production of benzophenone by phosgene are studied.

本文系统研究了光气路线生产二苯甲酮的合成技术、纯化方法及相应的清洁生产模式。

To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics.

研究人胎肝干细胞体外分离纯化方法，并对其进行初步鉴定及生物学特性分析。

To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics .

摘要研究人胎肝干细胞体外分离纯化方法，并对其进行初步鉴定及生物学特性分析。

In the argument between invention and discovery on patent of biotechnology .I ,firstly, take back look at the definition of invention and discovery in practice of patent of biotechnology in western countries . then ,analyse the possibility of putting the result of basic bioresearch into patent .Finally ,draw a conclusion :we should focus on the achievement of research on natural material and gene invention should be patented as long as the material and gene which have been separated and purified have real value .

关于生物技术专利中的发明与发现之争，本文首先回顾了西方发达国家在生物技术专利实践中对发明与发现的界定，接着分析了生物技术基础研究成果的可专利性，最后得出结论，我们应淡化发明与发现之分，着重于"三性"审查，即只要天然物质和基因被分离和纯化以后具有工业实用性，就应该给天然物质和基因发明等基础研究成果授予专利。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失