纯化

Crystallization was the key step in the process of extraction of amygdalin. Considering the purity and crystallization ratio of amygdalin, the amounts of ethanol and ether was chosen as 1:18 and 1:0.4, crystallization time was 36h.

结晶是分离、纯化苦杏仁苷的主要步骤，本文综合考虑结晶纯度和结晶得率，乙醇添加比为1：18，乙醚添加比为1：0.4 ，结晶时间选择36h。

They were able to greatly proliferateafter 3～4 days of cultureand from the cell clones having the analogic cell shape .

而如何得到大量纯化的骨髓MSCs则成为临床利用这些细胞治疗疾病的前提。

METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

In chapter 3, the animalcula of Dracaena cochinchinenis's wound were carefully isolated, it also argues the effect of fungi on the formation of the dragon's blood. Moreover, the loureirins of the liquid zymolysis products of some fungi were detected by HPLC, and the relationship between animalcula and the formation of the dragon's blood is further discussed.

第三章详细地论述了剑叶龙血树微生物群的分离、纯化及其对龙血竭形成的影响，并用HPLC对部分微生物的液体发酵产物作龙血素成分的检测，更进一步地了解微生物与剑叶龙血树龙血竭形成的关系。

Objective To study the purification technology of anthraquinone aglycone from Cassiae obtusifolia seeds by macroporous adsorption resin.

目的探索决明子蒽醌苷元的大孔吸附树脂纯化工艺。

The preparation process to extract and purity total anthraquinone was feasible.

该实验所确定的提取及纯化工艺对大黄总蒽醌的富集是切实可行的。

The invention relates to the isolation and preparation of an active fraction from plant Cuminum cyminum, its further purification and standardization as chemically defined entity with their intended use as drug bioavailability enhancer for the drugs belonging to therapeutic categories such as antimicrobial, antifungal, anti-viral, antitubercular, antileprosy, anti-inflammatory, antiarthritic, cardiovascular, antihistaminics, respiratory distress relieving drugs, immunosuppressants, anti-ulcerogenic, anti-cancer, CNS drugs, corticosteroids, nutraceuticals in compositions to be administered orally/parenterally, topically, inhalations, rectally, vaginally in human beings and/or veterinary conditions.

本发明涉及植物孜然芹中的一种活性级分的分离和制备，进一步的纯化以及它作为化学确定实体的标准化，和它作为治疗类药物的生物利用率增强剂的特定用途，这类药物包括如抗微生物剂、抗真菌剂、抗病毒剂、抗结核药、抗麻风病药、抗炎药、抗关节炎药、心血管类药、抗组胺药、呼吸窘迫缓解药、免疫抑制剂、抗溃疡药、抗癌药、CNS药、皮质甾类药、营养制品，它们以组合物的形式通过口服/肠胃外，局部，吸入直肠，阴道施用于人和/或兽医学病症中。

Objective To express and purify influenza virus M2 protein without transmembrane domain, and detect its antigenicity.

目的表达并纯化缺失跨膜区的流感病毒M2蛋白，并检测其抗原性。

Results: The titers of the antiserum is over 13000, The limit of detection is 1 ng/mL.

纯化后的抗体的效价大于13 000，最低检测限1 ng/mL。

A high level of expression of GST-NOR1 is obtained in JM 105, and its antiserum can be prepared successfully.

成功获得了高效表达NOR1基因的原核表达产物，通过对该融合蛋白的纯化为它的多抗制备奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失