纯化

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

Five mice of each group were challenged with 100 LD50 of Erysipelothrix rhusiopathiae virulent strain C43065 two weeks after the third immunization, and the specific antibody responses for SpaA was determined by indirect ELISA.

SDS-PAGE结果显示，采用GST Bind Resin纯化试剂盒和电洗脱法纯化得到了66 kDa的rSpaA-N和64 kDa的天然SpaA，蛋白含量分别为1.34 mg/mL 和1.26 mg/mL，而Western印迹结果表明rSpaA-N和纯化前后的SpaA具有良好的免疫反应性。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Objective: To choose the optimum resin for separation and purification of mistletoe flavonoids and to analyze influenceable factors of separation and purification process.

目的：筛选出分离纯化槲寄生总黄酮的最佳树脂，并对影响分离纯化的因素进行研究，得到优化的纯化条件。

In addition, our works also indicate that MC-ICPMS technique shows a good potential on Re-Os isotopic measurement. 2. A new method for the determination of low Re-Os abundances and ~(187)Os/~(188)Os in a natural rock sample by MC-ICP-MS has been established in our laboratory. Rock samples were digested in inverse aqua regia in sealed Carius tube for 24 hours at 230°C, then Os was separated from the matrix by CCl_4 solvent extraction, and further purification by microdistillation.

建立了Re-Os同位素样品的化学分离和纯化方法以及MC-ICPMS质谱测定方法：岩石样品的Re和Os化学分离方法是采用Carius管溶样法，结合四氯化碳萃取以及氢溴酸反萃取的方法分离出Os，最后是通过微蒸馏的方法纯化Os；利用阴离子交换树脂的方法分离纯化Re。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

Affinity chromatography materials for kinase;2. To search a suitable process route of purification of kinase in yeast by affinity chromatography, hexokinase was preparatorily purified by salt preciptation.

为确定一条采用亲和层析技术分离纯化酵母中磷酸激酶的较佳工艺路线，采用分步盐析初步纯化己糖激酶，考察硫酸铵饱争度对盐析效果的影响；以ATP为配基，SESA活化纸纤堆素制备亲和层析剂，确定最佳偶联条件和最佳静态吸附pH值后，将粗酶液直接上亲和层析柱，看分离纯化的效果；在载体和配基之问接上1。

The analysis of scanning electromicroscope showed that it didn't obviously exist protein granules in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased respectively after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

The analysis results of scanning electromicroscope showed that protein granules didn't obviously exist in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased remarkably after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失