纯化

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒，并对阳性病料做病毒分离与纯化。

The purity identification was made by SDS-PAGE,absorption spectrum and amino acid analysis.

SDS-PAGE电泳结果显示提取纯化的Ⅱ型胶原蛋白与S igm a公司的产品一致。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

METHODS Using heparin sepharose Cl-6B chromatography,the osteoinduction protein in reconstituted bone xenograftwas pu-rified and fractions obtained from purification were bioassayed for bone-induction activity by implantation into the thigh muscles of mouse.

采用肝素亲和层析技术纯化RBX中诱骨活性蛋白即骨形态发生蛋白，利用小鼠肌袋实验检测其骨诱导活性，再用聚丙烯酰胺电泳测量RBX中诱骨活性蛋白纯化后的相对分子质量。

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography.

对包涵体的复性条件进行了摸索，建立了IFN BLA的复性及纯化方法，纯化后的蛋白产量约为45mg/L，纯度在90%以上。

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography. The overall yield of IFN-BLA was about 45 mg/L with purity higher than 90%.

对包涵体的复性条件进行了摸索，建立了IFN-BLA的复性及纯化方法，纯化后的蛋白产量约为45 mg/L，纯度在90％以上。

Compared with the traditional method, this new method has some advantages:①centrifugation is not necessary, therefore, separation of high molecular weigh DNA is possible;②phenol, chloroform and other toxic organics are not needed;③separation is more efficient and less timeconsuming④the quality of DNA isolated in this way is very high.

当样品裂解液与杂交液1：1混合并用磁性微粒进行小麦黄化苗和种胚的DNA分离与纯化时，效果非常理想。与传统的纯化方法相比，该法具有①快速、简便；②无有机试剂污染；③无需离心分离；④得率和纯度均较高等优点。

The microspheres were used as solid phase adsorbents for extraction of genomic deoxyribonucleic acid from biological samples.

基于磁性微粒的核酸纯化方法就是其中的一种。利用磁分离技术进行核酸纯化通常要经历吸附、洗涤和脱附等3个步骤。

After cultured 8 days in vitro , the OECs were purified away from other cell types present in the cultures according to its biol...

实验结果发现，本研究采用的OECs纯化培养方法所获得OECs纯度在4个不同培养时间点都达到98%以上，说明此方法是一种高效率的纯化培养OECs的方法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失