纯化

Including the soybean lecithin separation purification, gamma - the linolenic acid separation and the purification, as well as gamma - linolenic acid fat 质体 Bao He studies and so on, and carries on optimal using the orthogonal experiment to the above craft.

其中包括大豆卵磷脂的分离纯化，γ-亚麻酸的分离和纯化、以及γ-亚麻酸脂质体包合研究等等，并利用正交试验对上述工艺进行优选。

On the other hand, Coarse goods ofβ-carotene was purified by macroporous resin and the purification parameters wasdetermined by single factor experiment. The effect of ray, heat, preservative and usualmetal ions to stability ofβ-carotene was also researched.

另外，利用大孔吸附树脂对β—胡萝卜素的粗提液进行了纯化，通过单因素试验确定了纯化的最佳工艺参数，同时，将光、热、常见防腐剂苯甲酸钠、常见金属离子对β—胡萝卜素的稳定性的影响进行了研究。

At first, we investigated the optimal components of culture medium for thereafter purification. We found that the optimal culture medium could produce better yield of red-pigmented antibiotic when 1.5% fructose and 1.5% malt extract included, and incubated at 30℃ under shaking conditions for 6 days.

我们逐步由探讨培养基条件为始，以利后续的生产纯化的进行，发现以1.5%果糖与1.5%麦芽抽出物之液态培养条件下，以30℃震荡培养6天后，有最适红色抗菌物质之产率，并且可减少其他色素的产生，再试著将其纯化。

Coli, and purify rWHcAg under native condition by precipitation with saturated ammonium sulfate and CsCl gradient centrifugation; Immunize Balb/c mice with purified rWHcAg to prepare antiserum; Establish a competitive inhibition ELISA method to detect WHcAb in serum of Chinese marmots.

原核表达重组WHcAg，氯化铯密度梯度离心获得非变性的纯化蛋白；用该纯化蛋白免疫Balb/c小鼠制备多克隆抗体；建立竞争抑制ELISA方法用于检测旱獭血清中的抗－WHc。

In general, the SERRS enhancement can also get a contribution from a so-called "chemical" SERS enhancement mechanism, which is based on the specific interaction between the target molecule and the metal substrate.

六。对单壁碳纳米管的纯化工作进行了讨论，我们采用了物理和化学方法相结合的方式来纯化单壁碳纳米管。

Results:We hae screened out the best method of drawing the materials from OB,which is crushing directly. we also conside the immunoadsorption as the best method of puring OECS.Coating the petri dish is profitable to pure the cells. meanwhile the appropriate cells density inoculated is important for eentual purity of culture.

结果：我们从中筛选出最佳取材方法为直接挤压法，最好的纯化嗅鞘细胞方法为免疫吸附法，同时发现包被培养皿对纯化细胞意义重大，而高密度接种细胞对提高细胞的最终纯度也是非常有利。

Objective: To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.

目的：研究大孔吸附树脂分离纯化三七花蕾中总皂苷的工艺条件及参数。方法：以三七花蕾总皂苷的吸附量和洗脱率为指标，筛选大孔吸附树脂，并研究所选树脂分离纯化三七花蕾总皂苷的吸附和洗脱条件。

It is a very important progress that a new procedure was developed using a re-formative gradient medium and by ultra-centrifugation for the isolation and purifi-cation of intact WSSV viral particles from the infected penaeid shrimp or crayfish.

在完整WSSV病毒粒子纯化技术上取得重要研究进展。采用改良的密度梯度离心介质建立了一套成熟的WSSV纯化方案。

Methods All mice in the experimental groups were immunosuppressed by given different concentrations of dexamethasone phosphate added in drinking water throughout the experiment. The recovery and purity of the oocysts obtained using different purification methods was compared. The infectivity of the oocysts obtained from the same origin but different animals and different purification methods in a bovine fallopian tube epithelial cell culture system was studied.

在饮水中添加不同浓度的地塞米松对人工感染小鼠进行免疫抑制，探讨获得最多卵囊数的药物添加量；比较不同纯化方法得到的卵囊回收率和纯度；探讨来自不同动物、不同纯化方法的卵囊在牛输卵管上皮细胞培养系统的感染情况。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失