纯化

The PCR production were purified by using Wizard PCR Preps DNA Purification Resin or Shrimp Alkaline Sequnencer.

PCR产物经树脂纯化或酶解纯化，在ABI377测序仪上进行测序。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示，用切向超滤法将病毒培养物浓缩为原体积的1/5后，再采用300g/L蔗糖垫底超速离心和300～600g/kg蔗糖密度梯度离心的方法进行纯化，可获得大量的纯化病毒粒子。

The unfolded protein correctly refolded to the native conformation through the column, and was partly purified during the elution process.

其原理是将层析技术应用于蛋白质复性和纯化，使变性蛋白质在层析柱上折叠为正确的空间构象，在洗脱的同时实现部分纯化。

The mixed microbial populations from the bioleaching plant in Zijinshan Copper Mine is separated and purified,and the identification based on the molecular biological,morphologic and physiologic methods of the microbe depurated and cultured are conducted by polyphasic taxonomy and phylogeny analysis with application of the 16S rDNA sequences and DNA homology technology.

对福建紫金山铜矿生物堆浸系统中的浸矿微生物进行分离纯化，对得到的纯化培养物采用了多相分类与系统发育分析方法，应用16S rRNA基因全序列分析技术，进行分子生物学鉴定、微生物形态性征与生理生化代谢特征鉴定。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

Further isomerization and substitution of this central intermediate leads to the synthesis of other flavonoids. CHSs has been isolated from different plant species, including both monocots and dicots, some of their genomic DNAs and/or cDNAs have also been isolated.

目前，包括单子叶和双子叶植物的很多物种中，有的物种查尔酮合成酶已经得到纯化且基因组、cDNA克隆得到分离，有的或者得到了纯化酶，或者分离得到了基因组、cDNA克隆。

Utilizing the reversed-phase high performance liquid chromatogram to carry out further purification to the deer placenta polypeptide, the best purifying condition of separation is: Chromatogram column Hypersil ODS C18 (4.6*250mm), A liquid 0.1% TFA /ACN, B liquid 0.1% TFA / water, in 30min10% A ～50% A and 90% B ～50% B linear gradient elutes, velocity of flow 1.0mL/min, temperature 30℃, detecting wavelength 214nm.

利用反相液相色谱技术对鹿胎盘多肽进一步纯化，最佳分离纯化条件为：色谱柱 Hypersil ODS C18(4.6×250mm )，流动相 A 液0.1% TFA/乙睛，B 液0.1%TFA/水，30min 内10%A ～50%A ，90%B ～50%B 线性梯度洗脱，流速1.0mL/min ，温度30℃，检测波长214nm 。

The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train.

本申请的特征还在于洗脱被纯化多肽的方法及纯化系列中该方法的并入。

Suitable macroporous adsorption resin (MAR DM-21) was chosen to separate SDG from extract solvent system (KOH-ethanol-water) of flaxseed cake.

然后用大孔吸附树脂与TLC相结合的方法，探讨了亚麻籽中SDG的分离纯化，并根据HPLC分析结果确定了最佳的分离纯化工艺条件。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失