纯化

Objective To study the conditions and effects of purification of the extract of Ligusticum chuanxiong Hort. by adopting macroporos resin.

目的 探讨大孔吸附树脂对川芎提取物的纯化条件及纯化效果。

The opposite application comprises: with Macleaya cordata fruit or grass as material and ethanol as extraction solvent, using just the adsorption-elution technique of one-step to separate and purify the high-pure sanguinarine from Macleaya cordata raffinate with purity more than 60%.

吸附树脂的应用是以博落回果或草为原料，以乙醇为提取溶剂，仅通过&吸附-洗脱&一步简单工艺，即可从博落回提取液中分离纯化得到高纯度血根碱的提取物，产品中由博落回果纯化得到血根碱的纯度最高可达60％以上。

The application effects of enzyme preparation in mashing process and kinetics for degradation of β-glucan in wort was studied.

探讨了双水相纯化β-葡聚糖酶工艺，筛选适宜的纯化体系，最后研究了β-葡聚糖酶在麦芽糖化过程中的应用效果，并建立了降解β-葡聚糖的动力学方程。

METHOUS: An aqueous extract of Rhizoma menispermi, an alcohol purified extract named phenolic alkaloids of menispermum dauricum and Rhizoma menispermi polysaccharide were isolated and purified by using the three-step method.

用三步法初步分离、纯化北豆根提取物，得到北豆根水提液(aqueous extract of rhizome menispermi， RMAE)、醇提液的初步纯化产物蝙蝠葛酚性生物碱(phenolic alkaloids of menispermum dauricum， PAMI)和北豆根多糖成分(rhizome menispermi polysaccharide， RMP)。

For Os separation, solvent extraction (Br_2 or CCl_4) and distillation are two mainly effective techniques, and microdistillation is used to further purify Os.

Os的分离纯化方法有蒸馏法和Br2或CCl4萃取法以及微蒸馏法，它们是分离和纯化Os的有效方法，也是目前国内外大多数实验室所采用的方法。

The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence ＋ serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁＋无血清条件培养液纯化的实验。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失