纯化

In order to simplify the procedure and obtain high quality gDNA, we alternate water-bath to dewax, extract gDNA from hepatocellular carcinoma embedded by paraffin, and amplify gene using HCC gDNA.

为了简化操作，获得更高质量的gDNA，我们采用水浴法替代二甲苯进行脱蜡，亲和层析纯化石蜡包埋肝细胞癌组织标本中基因组DNA，以纯化gDNA为模板PCR扩增看家基因。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

After collecting the bacterial cells and breaking the cell by means of ultrasonic, the inclusion bodieswere extracted. The extracted products were denatured with 8 mol/L urea and purified through Ni-NTAcolumn. The purified products were renatured by dialyzation, and then were analyzed by SDS-PAGE.

在优化条件下诱导重组菌表达，收集菌体经超声波破碎和裂解后提取不溶性包涵体，以8mol／L尿素处理包涵体提取物并进行Ni-NTA亲和层析纯化，纯化表达产物经透析复性后以SDS-PAGE分析其复性效果。

The active form of NGF is a dimer in which two monomer is linked by 3 disulphide bonds. This form of dimer makes the purification of NGF difficulty because the protein expressed in E.

在蛋白纯化过程中这种由二硫键形成的空间结构往往是蛋白纯化过程中复性的难点，尤其是在大肠杆菌表达生物蛋白时，所表达的蛋白往往以无活性的单体形式存在于细胞内。

The HBsAg in culture supernatant was purified by the same method, and their chromatographic and electrophoretic profiles were compared.

采用相同条件纯化培养上清中的HBsAg，比较二者的层析图谱及纯化抗原的电泳图谱。

It has been reported that NDP kinase has weak interaction with Hsp70, which can increase the ATP hydrolysis activity, so we carefully examined the possibility of NDP kinase contamination in the experiment. The equimolar mixture of NDP kinase and Hsp70 were digested and then 44-kD fragment was purified.

为获得准确的结论排除NDP激酶可能对实验的影响，按照纯化Hsp70 44-kD片段的方法对等物质量NDP激酶和Hsp70混合物进行酶切、纯化，检测得到的44-kD片段的ATP-ADP转换活性，确定Hsp70 ATP-ADP转换活性是Hsp70自身固有的，位于其N端44-kD片段处。

Genome, and its expression products are located in the flagellar, cytoplasm and fertilization tubule. Cr. actin is partially purified from Cr. cells by the method of assembly-disassembly-circle of actin.

我们利用肌动蛋白本身所具有的聚合和解聚的性质，从衣藻细胞中部分纯化了衣藻肌动蛋白，纯化的肌动蛋白能和抗兔α-平滑肌肌动蛋白的单克隆抗体发生专一性很强的免疫交叉反应。

OBJECTIES:: We report our obserations after Galanthus nialis affinity purification of recombinant HI enelope proteins (CHO gp120/140/160) deried from subtypes B/A and using these purified proteins as antigens in ELISA assays (against subtype C induced antibodies).

实验对象：来自B/A亚型的重组HI包膜蛋白（CHO gp120/140/160）经GN亲合纯化后，然后在ELISA试验中将这些纯化的蛋白作为抗原。

Keywords: diketoreductase,isomeride,separation and purification,statins chiral side-chain expression, photoaffinity labeling probe

通过DEAE-Sepharose离子交换柱及Sephadex分子筛柱纯化后，酶的纯度达到电泳纯，并通过正相硅胶柱和反相C-18柱分离纯化其反应产物。

The concentration of Jasminum flavonoids in the product was 11.63%- 13.02%, with developed nearly 10 times when compared with that of raw material.

纯化工艺放大实验能达到预期效果，纯化产品总黄酮含量为11.63％～13.02％，比原料中总黄酮含量提高了近10倍。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失