纯化

The average yield of AFP purified from an embryon was 0.7mg and the recovery of AFP was 45.71%. The efficiency of coupling measured by ultraviolet absorption method was 95.38%.The purified AFP was homogenous in SDS-PAGE. This method is suitable for scale manufacture of AFP.

本文报道用3～5个月胎龄的胚胎组织作材料，采用盐析、凝胶层析与亲和层析相结合的方法分离纯化AFP，以建立一种适合大批量生产AFP的工艺方法。

Alpha-fetoproteinhas been separated and purified from 3～5 months embryon by using salting out, gel chromatography and affinity chromatography.

甲胎蛋白（Alpha-fetoprotein，AFP）可从胎儿组织、胎儿心血、胎儿肝脏、羊膜液、脐带血清、肝癌患者血清和腹水等多种材料中分离获得，分离纯化方法国内外已有不少报道［1～6］。

All IBDV BC 6/85 F4 and IBDV HE BEI isolated strain were obtained from allantochonon and allantoic fluid of died embryonated eggs in 2-5 days after inoculating and virus-infected cell fluid that were frozen and thawed three times after approximately 80% of the monolayer exhibited cytopathic effects.

将纯化的IBDV分别免疫家兔和小鼠，制备了兔抗IBDV、鼠抗IBDV的高免血清，用健康兔的血清免疫羊而获得羊抗兔免疫血清。

Endocellular enzymes of Arthrobacter nicotinovorus D-97 showed a weak hydrolytic activity towards α-1, 4 linkage and glucose, maltose, sucrose and lactose could not be converted into trehalose.

综合采用薄层层析、高效液相电喷雾电离质谱联用(HPLC-ESI／MS)及核磁共振分析手段，在酶反应产物不必被纯化的前提下，最终证明食尼古丁节杆菌D-97对麦芽寡糖的酶反应产物中含有海藻糖。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

The putative endolysin gene was clone to Escherichia coli, and protein was expressed and purificated.

本实验将此预测之溶菌酶基因转殖於大肠杆菌，进一步表现与纯化此蛋白质。

The actinomyces was idicntified as a new species of the Microsircplosporu Its antifungal spectrum 024-antibiotic includes many plantpathogenic fungi, such as Glomerella cingulata, Fusarium gramiaearum, Helmmthosporiuin sativitnt,/iotrvosphticriu dothidea, Alternaria xolani, Endothia parasitica etc..

本文针对防治小麦纹枯病的上百种生防放线菌进行筛选后，选择出目标菌株024，随后对它的菌种的初步鉴定、初始发酵条件与最适发酵条件进行了探索，并对024放线菌所产抗生素的纯化、理化性质、发酵液的防治效果等内容进行了研究。

Injections containing glucos used as solvent is not suitable for this enzym

实验结果对进一步优化蒜酶的提取纯化工艺研究以及蒜酶的临床应用具有指导意义。

Objective The polysaccharides from fruiting bodies of Hericium erinaceus were isolated, purified, and characterized.

目的对猴头菇子实体中的多糖进行分离、纯化和组成分析。

Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test;2. After total saponins of Panax notoginseng were extracted,residues left was refined,separated,and purified.

通过对三七总皂苷提取后的药渣进行重新提取、分离与纯化得到了三七中另一有效成分———三七多糖，经含量测定可达50%，提高了三七的再开发利用价值。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失