纯化

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

Purified GO could oxidize both glycolate and glyoxylate, and both activities highly depended on FMN. The activity of glyoxylate oxidation was weaker than that of glycolate. During purification or storage of purified GO, the ratio of glycolate to glyoxylate oxidation was progressively enhanced.

纯化的菜心叶片乙醇酸氧化酶对乙醇酸和乙醛酸均有氧化活性，且两者都强烈依赖于FMN，但对乙醛酸的氧化能力相对较弱；在酶的纯化及纯化酶的贮存过程中其两催化活性的比值均呈逐渐升高的趋势。

Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶，比活性达130.9 U／mg，纯化倍数为137.4倍；纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰，且两者完全重叠；进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色，各在负极端出现一个活性斑点和蛋白染色斑点，且两者的位置完全对应；表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

To investigate the ability of ADA isozyme expression to discriminatebetweem lymphoid and nonlymphoid cell types in acute leukemia,the gelfiltration with FPLC system was used as micromethod for ADA isozymeanalysis.

为进一步阐明白血病细胞ADA同工酶生化性质，探讨制备ADA同工酶抗体的可能性，我们采用分子筛FPLC和阴离子交换FPLC系统二步法分离纯化健康人血清、单个核细胞和急性粒单核细胞白血病细胞ADA同工酶，获得部分纯化ADA1和ADA2，ADA1纯化71.62倍，活力62.2u/mg pr，ADA2纯化37倍，活力42.8u/mg pr，ADA总回收率44%，2步FPLC层析法酶活力回收率分别为68.4%和81.5%。

Large and medium scale air separation unit ; Molecular sieve purification system ; Adsorbent ; Uniform distribution ; Energy save

阐述了大中型空分设备分子筛纯化系统的技术新进展，详细分析了分子筛纯化系统设计以及运行中常见故障的预防和处理，对分子筛纯化系统设计制造方面的进步进行了展望。

Results Two months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P＜0.05),(38.8±13.7) pg/ml and (1.2±0.7) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are （72±18）%and 24±13）%,（65±21）%and 0 respectively after 1 and 2 months of transplantation.

结果移植后2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异（P.05），分别为(38.8±13.7) pg/ml和(1.2±0.7) pg/ml，纯化及未纯化海藻酸钠制备空囊移植组，植入1个月时微囊冲洗回收率分别为（72±18）%和（24±13）%，植入2个月时为（65±21）%和0。

The proteins were purified and used to prepare the rabbit antiserum against caprine IFN-γor IL-2,the results showed that the antibody could react with the purified proteins in Western blotting.

分别用ProBond~蛋白纯化试剂盒纯化，用所获得的重组蛋白纯化产物经三次背部皮下多点注射新西兰白兔，制备了兔抗山羊IFN-γ和兔抗山羊IL-2多克隆血清。

The results indicated that the purity and immunoactivity varied according to different purification methods. IgG purified by caprylic acid precipitation plus ammonium sulfate precipitation is the best for further use with 98.05%purity and 1800 ratio activity.

结果表明，不同方法纯化的抗体其纯度和免疫学活性具有一定程度的差别，其中经辛酸+饱和硫酸铵沉淀纯化的抗体为最佳，凝胶扫描纯度为98.05%，比活性近1 800，为纯化前的6.8倍。

The main conclusions are as follows:The vitellin of Chrysopa septempunctata was extracted with 0.4mol/L NaCl and then precipitated in cold distilled water. Ion exchange chromatography and proteic gel recovery were used to purify it further. SDS-PAGE analysis shows that the vitellin is composed of two subunits, the large one 165kDa and the small one 46kDa.Then automatic amino acid analyzer was used to analyse amino acid composition of vitellin.

主要结论如下：用蒸馏水沉淀法对大草蛉的卵黄蛋白进行了初步纯化，而后又采用离子交换层析和蛋白胶回收的方法对卵黄蛋白进行了进一步的纯化；通过SDS-PAGE分析表明，大草蛉的卵黄蛋白由大165kDa和小46kDa两个亚基组成；用氨基酸自动分析仪对纯化后的卵黄蛋白进行了氨基酸组分分析。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！