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纤维软骨

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RESULTS: The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes.

结果:实验兔后腿关节的股骨髁关节面的关节软骨缺损后,在制模后2,6周缺损部位凹陷明显,只有少许肉芽组织和纤维组织生长填充,未见软骨细胞爬行生长,12,18周见缺损部位以纤维组织增生为主,底部及边缘可见骨组织和软骨增生,但是镜下形态仍与正常软骨组织区别较大,表面仍未见软骨细胞爬行生长。

Six weeks after implantation, small amount of oval lacunae appeared. PGA fibers began to be absorbed. The amount of collagen increased and Safranin O stained positively. Eight weeks after implantation, cartilage with single or occasionally double chondrocytes in round lacunae was found.

体内埋植4周后可见PGA纤维间被不成熟的软骨细胞充填。6周时出现椭圆形软骨陷窝,PGA纤维开始吸收,胶原增多,番红花—"O"有阳性染色。8周时软骨陷窝呈圆形,单个,双个对称或多个排列。

Results: Arthroscopic examination showed that there were synovitis, debris and fibrillation in the joints of ADD with reduction; synovitis, synovial hyperplasia, debris, fibrillation, chondromalacia,fibrocartilage rupture and adhision in the joints of ADD without reduction.

结果:可复性关节盘前移位的病例出现滑膜炎,关节腔内有絮状物,关节结节表面有纤维形成。不可复性关节结节表面纤维形成,关节软骨软化,软骨剥脱,同时伴有纤维粘连。

In this study, immunohistochemical staining was performed with antibodies against D2-40, S100, pankeratin, epithelial membrane antigen, brachyury, and glial fibrillary acidic protein in 4 cases of chordoid glioma, 6 skeletal myxoid chondrosarcomas, 10 chordoid meningiomas, 16 extraskeletal myxoid chondrosarcoma, 18 chordomas, 22 low-grade chondrosarcomas, and 27 enchondromas.

本研究中,我们给4例脊索样胶质瘤、6例骨的黏液样软骨肉瘤、10脊索样脑膜瘤、16例骨外黏液样软骨肉瘤、18例脊索瘤、22例低级别软骨肉瘤和27例内生性软骨瘤做了D2-40、S100、pankeratin、上皮膜抗原、brachyury和胶质纤维酸性蛋白的免疫组化染色。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富,软骨基质染色均匀,软骨细胞数目最多,Ⅱ型胶原蛋白表达最活跃,平均积分光密度值最高;青少年组软骨膜内血管减少,软骨基质染色出现明显的不均质状,软骨陷窝体积变大,并呈分隔状,陷窝内软骨细胞数目减少,II型胶原蛋白表达较儿童组减弱;成人组软骨膜血管、细胞成分明显减少,软骨膜内的纤维成分明显玻璃样变,钙盐沉积较青少年组时明显增多,Ⅱ型胶原蛋白表达较青少年组减弱。

METHODS: A total of 50 white rabbits were randomly divided into anterior cruciate ligament transection group, medial collateral ligament transection + meniscectomy group and normal control group. The specimens were harvested at weeks 1, 2, 3, 4, and 5 after operation. The osteocartilaginous section of the medial femur condyles were observed by gross observation, histological examination, and Markin scores. The contents of proteoglycan and collagen fiber were also measured.

取大耳白兔50只,随机分为3组前交叉韧带切除组、内侧副韧带+半月板切除组分别切除右后肢前交叉韧带、右后肢内侧副韧带和半月板,正常对照组不干预,分别在建模1,2,3,4和5周后分批于膝股骨内髁软骨取材,进行软骨组织大体和组织学观察、软骨损伤程度Markin评分;测定软骨组织蛋白多糖及软骨基质中胶原纤维含量。

Immunofluorescence cytochemistry was used to detect the expression of IPSCs fibroblast growth factor receptor 3, the expressions of SV40Tag and fibroblast growth factor receptor 3 in the human precartilaginous stem cells were determined by RT-PCR.

以免疫荧光细胞化学技术检测永生化人前软骨干细胞成纤维生长因子受体3,以RT-PCR检测永生化人前软骨干细胞及人前软骨干细胞中SV40Tag和成纤维生长因子受体3表达。

In electricity plus thermal group, the pathologic alterations were slighter than model group; there was little crack on the surface of cartilage, with intact collagen fiber structure and less pyknotic chondrocytes. Although some chondrocytes shrank, there were intact cell organs in many chondrocytes even chondrocyte cluster in some regions.

疏密波+热软膜组在病理程度上的改变明显轻于模型对照组,软骨表面裂纹少,胶原纤维结构基本完整,固缩的软骨细胞少,虽可见部分退变的软骨细胞,但部分软骨细胞具有较多的细胞器,某些区域形成软骨细胞簇。

During operation, an intranasal and marginal combining incision was made to expl ore the alar cartilages and to from a mucosal-cartilage flap in the nasal vest ibule. After the deformity of septal cartilage and the abnormalities of the alar base on the cleft side were repaired, the total alar cartilage was repositioned and rotated with suspension and V-Y advancement to correct the nasal deformiti es. Results Since 1993, a total of 92 cases were treated by the above p rocedure and satisfactory results were obtained.

手术经鼻端和患侧前庭联合切口,解剖、显露双侧鼻翼软骨,同时形成患侧前庭粘膜软骨瓣;在纠正鼻中隔软骨偏曲,松解患侧鼻肌复合体起点,使鼻翼脚无张力抬高后,再通过悬吊技术和粘膜软骨瓣的V-Y推进,将患侧鼻翼软骨进行整体旋转复位;最后,应用口轮匝肌上部纤维内收抬高鼻翼脚,用鼻肌复合体起点复位调整鼻孔形态,完成鼻畸形的矫正。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果:①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后,逐渐呈灰黄色,表面粗糙,出现裂隙;当压力逐渐减小后,变黄、粗糙、有裂隙的软骨颜色逐渐恢复,变得有光泽②光镜下可见表面光滑、平整,细胞分布均匀、整齐,层次清楚的软骨在关节面压力增大后,表面变粗糙、缺损,细胞排列紊乱、染色不均;当关节面压力逐渐减小后,软骨表面逐渐修复,细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达,术后3周内表达逐渐变弱,从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后,软骨细胞逐渐变扁,胞质中内质网膜、高尔基体减少,胶原排列紊乱;当关节面压力减小,软骨细胞形态逐渐恢复正常,胞质中内质网膜、高尔基体数量逐渐恢复;胶原纤维排列逐渐有规则。

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